相关实验视频

Updated: Jul 5, 2025

Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix

Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix

Published on: February 9, 2015

带有抗生素的PMMA间隔剂对hMSCs骨质生成潜力的影响

Jakob Hofmann¹, Tim Niklas Bewersdorf¹, Ulrike Sommer¹

¹Clinic for Trauma and Reconstructive Surgery, Centre for Orthopedics, Trauma and Reconstructive Surgery and Paraplegiology, University Hospital Heidelberg, Schlierbacher Landstrasse 200a, 69118 Heidelberg, Germany.

Antibiotics (Basel, Switzerland)

|January 22, 2024

概括

用抗生素加载的骨水泥与甘他米辛和万科米辛显示出细胞毒性,但没有损害骨愈合. 然而,Gentamicin加Clindamycin在体外显著降低了细胞数量和骨质生成潜力.

科学领域:

整形外科手术整形外科手术
生物材料科学生物材料科学
干细胞生物学干细胞生物学

背景情况:

带有抗生素的聚甲基甲酸 (PMMA) 骨在骨科手术中被广泛使用.
虽然一些抗生素表现出细胞毒性,但临床结果表明对骨愈合没有不良影响.
研究这些水泥对骨质生成的体外影响至关重要.

研究的目的:

为了评估带有抗生素的PMMA间隔剂在体外骨质生成的影响.
评估Gentamicin,Vancomycin及其组合对人类骨髓中介细胞干细胞 (BM-hMSCs) 的影响.

主要方法:

培养的BM-hMSCs与PMMA间隔器含有仁胺,万科米辛,仁胺+克林达米辛,仁胺+万科米辛,或没有抗生素 (控制).
通过DAPI染色来评估细胞号码.
通过基亚帕提特合成 (Tc-HDP标记<99m) 和细胞培养超级生物中的/酸盐水平来测量骨质生成潜力.

主要成果:

单独和组合使用的珍塔和万科米显示出一些细胞毒性,但没有对骨质产生潜力产生负面影响.
亨塔米辛和克林达米辛的组合导致细胞数量和骨质性潜力显著下降.
对照组 (糖和无抗生素的PMMA) 没有显示任何不良影响.

结论:

尽管有一些细胞毒性,但PMMA骨质水泥中的甘他米辛和万科米辛在体外骨质生成中似乎是安全的.
由于Gentamicin和Clindamycin对骨质生成的有害影响,因此应谨慎使用Gentamicin和Clindamycin的组合.
这些发现支持了对某些带有抗生素的骨进行良好的骨愈合的临床观察.

关键词:

在99mTc-HDP标签上在PMMA骨头水泥.抗感染剂是一种抗感染剂.骨缺陷是一种骨缺陷.克林达米辛是一种这是一种 gentamicin 药物.没有工会的非工会.骨质发生过程 (osteogenesis)这就是Vancomycin.

更多相关视频

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells

Published on: May 4, 2018

Fabricating Highly Open Porous Microspheres HOPMs via Microfluidic Technology

Fabricating Highly Open Porous Microspheres HOPMs via Microfluidic Technology

Published on: May 16, 2022

相关概念视频

Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...