在两相培养过程中,在转基因大肠杆菌中产生 styrene
Shuhei Noda1,2, Ryosuke Fujiwara3, Yutaro Mori4
1Graduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan.
Biotech (Basel (Switzerland))
|January 22, 2024
概括
研究人员利用大肠杆菌 (Escherichia coli) 产生烯,这是一个关键的工业化学品. 这种工程菌株在批量培养中实现了创纪录的 styrene 产量,为工业规模生产铺平了道路.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 工业微生物学 工业微生物学
背景情况:
- styrene 是一种重要的工业化学品,市场需求很大.
- 以前的微生物烯生产方法在产量和可扩展性方面存在局限性.
- 在微生物宿主中增强烯生物合成对于可持续的工业应用至关重要.
研究的目的:
- 为了设计一种微生物菌株以增加 styrene 产量.
- 为了优化烯前体的生物合成途径,转酸 (Cin).
- 为了在使用转基因大肠杆菌的批次培养中实现高产烯生产,使用转基因大肠杆菌.
主要方法:
- 从Arabidopsis thaliana (AtPAL) 和Brachypodium distachyon (BdPAL) 中选五种氨酸酶 (PAL),用于跨酸的生产.
- 一种联合表达AtPAL2和Saccharomyces cerevisiae铁素酸脱碳酶的大肠杆菌菌株的构建.
- 使用醇作为有机溶剂来增强烯回收的双相栽培.
主要成果:
- 在大肠杆菌中AtPAL2的表达产生了大约700mg/L的跨酸.
- BdPALs 证明了将跨酸转化为 styrene 的能力.
- 改造的大肠杆菌菌株从批量培养中从葡萄糖中获得了3.1 g/L的 styrene 生产,产量为26.7% (mol/mol).
- 这些结果代表了批量种植中烯生产的最高报告值.
结论:
- 开发的基因工程大肠杆菌菌株对 styrene 生物合成非常有效.
- 取得的生产水平适合潜在的大规模工业化 styrene 制造.
- 这项研究在微生物生产烯方面取得了重大进展,为传统方法提供了可持续的替代方案.
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