感染寨卡病毒的单细胞外体介导细胞间的病毒传播
Pedro Pablo Martínez-Rojas1, Verónica Monroy-Martínez1, Lourdes Teresa Agredano-Moreno2
1Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad de México 04510, México.
Cells
|January 22, 2024
概括
寨卡病毒 (ZIKV) 可以通过受感染单细胞释放的外体传播. 这些外体促进ZIKV的传播,感染和细胞激活,为严重的寨卡病毒疾病提供了新的理解.
科学领域:
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
背景情况:
- 寨卡病毒 (ZIKV) 是一种由关节动物传播的病毒,导致严重的神经和发育障碍.
- 单细胞是ZIKV的关键目标,它们的反应包括释放外体细胞.
- 外基因组在病毒感染期间在细胞间通信和免疫逃避中发挥作用.
研究的目的:
- 调查来自ZIKV感染单细胞的外体在病毒传播中的作用.
- 确定这些外体是否可以调解ZIKV.的细胞间传播.
主要方法:
- 用差异超离心法从感染ZIKV的人类单细胞 (Mø exo ZIKV) 中分离出外体.
- 通过纳米粒子跟踪分析,传输电子显微镜和表面标记物检测 (CD63,CD81,TSG101,Alix) 对外体的表征.
- 净化外体,评估它们的传染性和对受体细胞的影响.
主要成果:
- 据证实,Mø exo ZIKV 含有 ZIKV RNA 和 E/NS1 蛋白.
- 对原始细胞暴露于Mø exo ZIKV促进了病毒传播和感染.
- 与Mø exo ZIKV的相互作用导致受体细胞的分化和激活.
结论:
- 单细胞衍生外体作为ZIKV细胞间传播的有效途径.
- 了解这种传播途径对于ZIKV病原性研究至关重要.
- 这些发现可以为新型ZIKV疫苗和治疗方法的开发提供信息.
相关概念视频
00:57Viral Recombination
23.4K
Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
23.4K
01:36Overview of Exosomes
2.7K
Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
2.7K