在人体巨细胞,基细胞和氨细胞中的冠状病毒受体表达特征
Lina Degenfeld-Schonburg1,2, Irina Sadovnik1,2, Dubravka Smiljkovic1,2
1Department of Internal Medicine I, Division of Hematology & Hemostaseology, Medical University of Vienna, 1090 Vienna, Austria.
Cells
|January 22, 2024
概括
mast细胞,基细胞和乙酸细胞表达不同的冠状病毒受体 (CoV-Rs),包括CD13和CD147. 这些发现可能为SARS-CoV-2病原和炎症提供新的见解.
科学领域:
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
- 细胞生物学 细胞生物学
背景情况:
- SARS-CoV-2 感染会导致严重的炎症,特别是在肺部.
- 乳腺细胞 (MC),基细胞 (BA) 和乙素细胞 (EO) 是关键的免疫效应细胞,参与炎症.
- 这些细胞最近与SARS-CoV-2的病变发生有关.
研究的目的:
- 调查人类初级MC,BA,EO和相关细胞系上冠状病毒受体 (CoV-Rs) 的表达特征.
- 为了确定常见的抗炎药物是否会影响这些细胞上的Cov-R表达.
主要方法:
- 使用流细胞计测试来检测CoV-R蛋白表达在初级细胞和细胞系上.
- 使用定量PCR (qPCR) 来评估Cov-R mRNA转录.
- 细胞被用甲松,维生素D和化治疗,以评估药物对Cov-R表达的影响.
主要成果:
- 主要的MC,BA,EO及其细胞系表达了CD13和CD147.
- CD26在初级皮肤MC和BA以及EOL-1细胞上表达,但不在初级EO或大多数MC/BA细胞系上表达.
- 在大多数细胞系中,mRNA分析揭示了CD13,CD147和ABL2转录,其中ACE2mRNA无法检测,CD26mRNA只在EOL-1细胞中.
- 德甲,维生素D和氧化没有显著改变CD13,CD26或CD147的表达.
结论:
- 乳腺细胞,基细胞和乙酸细胞表现出独特的冠状病毒受体表达特征.
- 已识别的CoV-Rs (CD13,CD147,CD26) 可能在SARS-CoV-2与这些免疫细胞的相互作用中发挥作用.
- 需要进一步的研究来确认这些受体是否调解病毒细胞相互作用并导致SARS-CoV-2引起的炎症.
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