卡尔西蛋白颗粒诱导人类动脉内皮细胞的细胞区特异性蛋白质组变化
Daria Shishkova1, Arseniy Lobov2, Egor Repkin3
1Department of Experimental Medicine, Research Institute for Complex Issues of Cardiovascular Diseases, 6 Sosnovy Boulevard, 650002 Kemerovo, Russia.
Journal of cardiovascular development and disease
|January 22, 2024
概括
蛋白颗粒 (CPPs) 触发细胞应激反应和内皮细胞 (ECs) 中的亡. 这项研究揭示了CPPs改变ECs的蛋白质组,影响信号传输,运输和能量代谢.
科学领域:
- 心血管生物学 心血管生物学
- 细胞蛋白质组学 细胞蛋白质组学
- 生物矿物化 生物矿物化
背景情况:
- 蛋白颗粒 (CPPs) 清理血液中的多余和离子.
- 内皮细胞 (ECs) 内化和处理CPPs,但它们的分子反应尚未完全理解.
研究的目的:
- 调查无形 (CPP-P) 和晶体 (CPP-S) 蛋白颗粒对人类冠状动脉和内胸动脉ECs.s蛋白质的影响.
- 为了识别EC中CPPs诱导的有机体特异性路径改变.
主要方法:
- 在人类初级冠状动脉ECs (HCAEC) 和人体内胸动脉ECs (HITAEC) 中对细胞区特异性蛋白质体 (细胞核,核,线粒体,ER) 的途径丰富分析.
- 用CPP-P和CPP-S.处理EC的方法
- 线粒体和核溶解物的蛋白质组学分析.
主要成果:
- CPP暴露上调了细胞因子/化学因子信号传递,Ca2+依赖事件,以及细胞因子/核蛋白质组中的亡.
- 线粒体蛋白质体显示H+/Ca2+运输,ROS生成和亡的增加,以及能量代谢的减少.
- ER蛋白质体表现出氧化,和ER压力,并具有上调的亡.
- 转录,翻译,细胞循环和细胞粘附途径的下调,在细胞/核区观察到.
结论:
- CPPs诱导显著的细胞应激,细胞亡和内皮细胞中的代谢重编程.
- 在不同EC类型和CPP形式 (CPP-P与CPP-S) 中,有机细胞特异性蛋白质变化是一致的.
- 研究结果强调了CPP作为内皮细胞功能和活力的强大调节剂.
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