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在重症监护室爆发Aspergillus:来源分析与全基因组测序和短并列重复

Stephan J P Hiel¹, Amber C A Hendriks^2,3, Jos J A Eijkenboom¹

¹Department of Intensive Care, Máxima Medical Centre, De Run 4600, 5504 DB Veldhoven, The Netherlands.

Journal of fungi (Basel, Switzerland)

|January 22, 2024

概括

全基因组测序 (WGS) 有效地调查了在重症监护室的阿斯伯吉卢斯疫情. 该研究没有发现分离物之间的遗传相似性,这表明环境子积累而不是单一来源.

科学领域:

医学真菌学医学真菌学
传染病流行病学传染病流行病学
基因组监测基因组监测

背景情况:

全基因组测序 (WGS) 在真菌学中未被充分利用,用于疫情调查.
重症监护病房 (ICU) 容易感染真菌,特别是在施工期间.
在医疗保健机构中,Aspergillus fumigatus构成重大风险.

研究的目的:

在疑似ICU爆发期间,在Aspergillus fumigatus分离物基因定型中应用WGS.
为了确定临床和环境Aspergillus分离物的遗传相关性.
调查阿斯伯吉勒斯疫情的来源和传播动态.

主要方法:

30个Aspergillus fumigatus分离物的全基因组测序 (WGS) (6个患者,4个环境,20个无关).
Illumina NextSeq 550用于测序. 这是一个很好的方法.
基于SNP的家族遗传树的构建和对比分析与短并列重复 (STRs).

主要成果:

六名患者和四名环境隔离物与疫情有关.
对比分析显示,与疫情相关的菌株之间没有遗传相似性.
在WGS和STR分析中,Aspergillus fumigatus的菌株多样化,而不是单一的克隆来源.

结论:

全基因组测序是分析Aspergillus fumigatus在爆发环境中的菌株间关系的合适方法.
阿斯珀吉勒斯病毒的爆发很可能是由建筑过程中环境中子释放引起的,而不是单一的持续来源.
停止建筑活动导致了在ICU中解决新的Aspergillus病例.

关键词:

亚斯伯吉拉斯烟虫 (Aspergillus fumigatus) 是一种有毒的植物.建筑工程建筑工程建筑工程源头分析源头分析整个基因组的测序.

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