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在固态发酵中优化碳和,以利用工业废物可持续生产脂

Estefanía Eras-Muñoz¹, Teresa Gea¹, Xavier Font¹

¹Department of Chemical, Biological and Environmental Engineering, Escola d'Enginyeria, Composting Research Group (GICOM), Universitat Autònoma de Barcelona, Barcelona, Spain.

Frontiers in bioengineering and biotechnology

|January 22, 2024

概括

这项研究优化了固态发酵,以利用工业废物可持续生产脂 (SL). 在最佳条件下,可获得高乙酸乳C18:1的产量,证明了废物价值化潜力.

科学领域:

生物技术和生物工程生物技术和生物工程
可持续化学可持续化学
工业微生物学工业微生物学

背景情况:

脂 (SLs) 是微生物生物表面活性剂,具有多种应用.
目前的生产方法,如浸泡发酵 (SmF),对利用固体废物有局限性.
固态发酵 (SSF) 为提升工业和食品废物的价值提供了一个有希望的替代方案.

研究的目的:

优化固态发酵 (SSF) 以利用工业废物增强脂 (SL) 生产.
研究碳和来源对酵母生长和SL生产的影响.
重新利用工业有机废物作为SL生物合成的可持续原料.

主要方法:

在实验室规模的SSF系统中,利用冬季化油蛋糕作为疏水性碳源.
采用Box-Behnken统计设计与响应表面方法来优化葡萄糖和尿素的比率.
分析了时间过程发酵,并用工业废物 (泥,水解剂,废水) 代替纯基质.

主要成果:

最佳条件 (葡萄糖:比为181.7:1.43,发酵100小时) 最大化了二甲基化乳C18:1产量 (0.54g),产量为0.047g/g.
在优化条件下,与参考条件相比,原始SL提取物增加了22%,二甲基化乳C8:1增加了30%.
工业废物,包括糖果行业的废水和化品污泥,作为有效的碳和来源,达到约70%的控制产量.

结论:

固态发酵是从工业废物中可持续生产脂的有效策略.
优化的碳和来源比率显著提高了SL产量和生产力.
工业有机废弃物流可以成功地被重新利用为索法脂生物合成的替代原料.

关键词:

生物转化生物转化生物表面活性剂生物表面活性剂化品行业化品行业实验设计实验设计废弃物再价值化的残留物固态发酵发酵的固态发酵方式这就是所谓的sophorolipid.

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