在确定TACE有效性中GNMT和MMP12表达的作用:转录和蛋白质水平的验证
Tianhao Cong1, Chao Yang1, Qi Cao2
1Department of Interventional Therapy, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of China.
Journal of hepatocellular carcinoma
|January 22, 2024
概括
一个新的2基因特征,甘氨酸N-甲基转移酶 (GNMT) 和矩阵金属蛋白酶-12 (MMP12),预测了肝细胞癌 (HCC) 中的跨动脉化学栓塞 (TACE) 反应. 失去GNMT和高MMP12表明TACE的有效性很差.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物标志物 生物标志物
背景情况:
- 肝细胞癌 (HCC) 是一种主要的肝癌.
- 超动脉化学栓塞 (TACE) 是HCC的关键治疗方法.
- 预测TACE反应对于患者管理至关重要.
研究的目的:
- 开发一种基因特征,以预测HCC患者的TACE反应.
- 确定TACE治疗疗效背后的分子机制.
主要方法:
- 使用了三个独立的HCC数据集 (GSE104580,GSE14520,外部验证).
- 使用后勤回归开发了一个2基因签名 (GNMT,MMP12).
- 通过免疫组织化学证实基因表达.
- 进行了免疫透和功能丰富分析.
主要成果:
- 一个2基因签名 (GNMT和MMP12) 有效地将患者分为高 (HE) 和低 (LE) TACE有效性组.
- 高血压组显示出明显更好的预后 (OS,PFS) 比LE组.
- 在LE组中观察到GNMT的损失和MMP12的过度表达.
- 独立地预测了较差的生存结果 (OS,DFS,PFS).
- 在LE组中,增加了M0巨细胞和激活的巨细胞,以及丰富的缺氧和糖解路径.
结论:
- 2基因特征 (GNMT/MMP12) 准确地预测了HCC中TACE反应.
- 丧失GNMT和MMP12过度表达是影响TACE疗效的关键因素.
- 免疫透和代谢途径可能是TACE耐药性的基础.
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