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在成年老鼠嗅觉上皮质中,ATP的构成性和唤起释放

Sébastien Hayoz¹, Cuihong Jia², Colleen Cosgrove Hegg³

¹Department of Physiology, University of Arizona, Tucson, Arizona 85724, USA.

Open life sciences

|January 22, 2024

概括

成人的嗅觉上皮表现出持续的ATP释放,这对组织修复和再生至关重要. P2X7受体在轮中调解ATP释放,突出了嗅觉神经元恒温的机制.

科学领域:

神经科学是一个神经科学.
细胞生物学细胞生物学
生理学生理学生理学

背景情况:

成人的嗅觉上皮质 (OE) 依赖ATP进行细胞循环和再生.
之前的研究表明,新生儿OE中的ATP释放涉及P2X7受体.
大人的OE中ATP释放的作用在很大程度上仍未被探索.

研究的目的:

调查成年老鼠OE中的构成性和唤起的ATP释放.
为了确定嗅觉感官神经元对ATP释放的贡献.
确定参与成人OE中ATP释放的纯能受体.

主要方法:

使用了嗅觉感官神经类似的OP6细胞和初级成年OE培养.
采用成像来评估纯能受体功能.
使用HEK细胞生物传感器和 luciferin-luciferase测试监测ATP释放.

主要成果:

在OP6细胞和成年OE轮体中检测到构成性ATP释放.
由P2X7受体介导的诱发ATP释放,特别在成年OE轮胎中观察到.
在OP6细胞和初级成年OE培养物中证实了功能性纯能受体.

结论:

ATP释放机制有助于成年人OE稳态和受伤后再生.
对这些ATP释放通路的进一步研究是有必要的,以了解OE组织维护.
研究结果提供了关于纯能信号在嗅觉系统中的生理作用的见解.

关键词:

的OP6细胞.与焦点一致的成像技术免疫组织化学免疫组织化学 luciferin 原始细胞培养是一种细胞培养.

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