莱什曼尼亚菌种分子类型的分类 在意大利东北部引起体莱什曼病,2014-2020年
Tommaso Gritti1, Elena Carra2, Gert Van der Auwera3
1Department of Medical and Surgical Sciences, University of Bologna, 40126 Bologna, Italy.
Pathogens (Basel, Switzerland)
|January 22, 2024
概括
意大利的体莱什曼病 (TL) 越来越多地是由独特的莱什曼病婴儿变体引起的. 这项研究在意大利东北部发现了这些新兴菌株,突出显示了当地寄生虫种群的遗传多样性.
科学领域:
- 医学寄生虫学 医学寄生虫学
- 分子流行病学分子流行病学
- 传染性疾病 传染性疾病
背景情况:
- 体莱什曼病 (TL) 是南欧的一个被忽视的流行病.
- 意大利东北部最近有TL病例的增加.
- 了解循环的Leishmania物种及其遗传多样性对于疾病控制至关重要.
研究的目的:
- 为了识别和描述意大利东北部TL的负责Leishmania菌株.
- 调查该地区Leishmania种群中的遗传变异性.
- 评估新型Leishmania变种的出现情况.
主要方法:
- 来自甲固定,嵌 (FFPE) 组织活检的DNA分析.
- 核糖体内部转录间隔器1 (ITS1) 和热冲击蛋白70 (hsp70) 基因的放大和测序.
- 在Leishmania donovani复杂基因组的分析中.
主要成果:
- 80.7%的样本成功地被类型化,在大多数本土病例 (91.8%) 中识别了Leishmania infantum.
- 在很少的病例中检测到Leishmania major和Leishmania tropica,这些病例可能是进口的.
- hsp70类型检测显示了八种不同的Leishmania donovani复杂变体,在当地L. infantum种群中具有很高的遗传变异性.
结论:
- 在意大利东北部的TL的新焦点中,奇特的Leishmania婴儿变体正在出现.
- 这些发现表明该地区Leishmania种群中的遗传多样性很大.
- 这凸显了在新兴的特有地区对莱什曼尼亚菌株的持续监测和表征的需要.
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