相关实验视频

Updated: Jul 5, 2025

Sexual Development and Ascospore Discharge in Fusarium graminearum

Sexual Development and Ascospore Discharge in Fusarium graminearum

Published on: March 29, 2012

松树癌病原体Fusarium circinatum的染色体水平组合

Lieschen De Vos¹, Magriet A van der Nest², Quentin C Santana³

¹Department of Biochemistry, Genetics and Microbiology (BGM), Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria (UP), Pretoria 0002, South Africa.

Pathogens (Basel, Switzerland)

|January 22, 2024

概括

高质量的Fusarium circinatum基因组组合,主要的松树病原体,揭示了可用染色体的显著变异性. 这种与重复元素和中间体相关的变异性,为病原体进化和染色体结构提供了洞察力.

科学领域:

植物病理学植物病理学
基因组学就是基因组学.
菌类学菌类学是指菌类学.

背景情况:

Fusarium circinatum是全球松树林的重大威胁.
现有的F.circinatum基因组序列是碎片化的,缺乏结构定义.

研究的目的:

为了生成高质量的染色体级基因组组件,用于两个F.circinatum菌株.
为了描述编码内容,重复元素,端粒和中心粒.
研究基因组变异性,特别是在可用染色体中.

主要方法:

利用了牛津纳米孔技术的MinION长读和Illumina短读测序.
采用基因组合成来实现从端粒到端粒的染色体层次组合.
对基因组结构和内容进行了比较分析.

主要成果:

获得了染色体水平的组合,显示出高度的连续性.
在可用的第12染色体中确定了实质性的长度多态和变异性.
在可变染色体区域观察到不同的特征 (较低的基因密度,G+C含量,更高的重复流行率).
发现了与染色体内差异和断点相关的中心元素.

结论:

这项研究为F. circinatum的研究提供了重要的,高质量的基因组资源.
可用染色体的变异性,可能是由可转移元素驱动的,为基因组可塑性提供了洞察力.
中心分子可能在塑造F.circinatum染色体结构方面发挥作用.

关键词:

中心的中心 (centromere)没有必要的染色体.在染色体内转位.端粒是什么意思端粒是什么意思

更多相关视频

High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization

High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization

Published on: June 28, 2012

A Fast Air-dry Dropping Chromosome Preparation Method Suitable for FISH in Plants

A Fast Air-dry Dropping Chromosome Preparation Method Suitable for FISH in Plants

Published on: December 16, 2015

相关概念视频

Genome Annotation and Assembly

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.

Assembly of Cytoskeletal Filaments

Assembly of Cytoskeletal Filaments

Cytoskeletal filaments are polymeric forms of smaller protein subunits. However, individual cytoskeletal filaments may easily disassemble or associate with other similar filaments to form rigid structures. Microfilaments, made of actin monomers, rely on actin-binding proteins to form bundles and create networks of individual actin filaments. Microtubules rely on microtubule-associated proteins (MAPs) to form sturdy cylindrical structures. However, the proteins involved in forming complex...

Histone Variants at the Centromere

Histone Variants at the Centromere

Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...