使用MALDI-TOF与被困离子移动性检测,定量和分离异构体的Per-和多基物质 (PFAS) 的MALDI-TOF
Journal of the American Society for Mass Spectrometry
|January 22, 2024
概括
一种新方法将MALDI-TOF-MS与TIMS相结合,用于对和多醇基物质 (PFAS) 的快速分析. 该技术实现了敏感检测和异构体分离,有助于环境和健康研究.
科学领域:
- 环境化学环境化学
- 分析化学 分析化学
- 质谱测量质量谱测量
背景情况:
- 和多基基物质 (PFAS) 是持久性环境污染物,已知对健康有不良影响.
- 准确的分析方法对于在各种样本矩阵中表征PFAS至关重要.
- 矩阵辅助激光脱/电离质谱法 (MALDI-MS) 为现场样本分析提供了无染色学方法.
研究的目的:
- 开发和优化一种用于敏感和定量PFAS检测的新型分析方法.
- 通过将被困离子移动性光谱学 (TIMS) 与MALDI-MS集成来增强PFAS的表征.
- 为了证明开发的方法对异构体分离和高通量分析的能力.
主要方法:
- 使用矩阵辅助激光脱/离子化飞行时间质谱 (MALDI-TOF-MS) 与被困离子移动性谱 (TIMS) 相结合.
- 优化了MALDI矩阵组成和仪器参数,用于校准曲线生成.
- 采用离子移动性频谱过,以提高灵敏度和分离.
主要成果:
- 实现了各种 perfluorosulfonic 酸 (PFSA) 和 perfluorocarboxylic 酸 (PFCA) 的每十亿分之一 (ppb) 校准曲线.
- 用离子移动性过来证明PFSAs的每万亿分之一 (ppt) 校准曲线.
- 通过TIMS.在气相中成功分离了三种完全甲硫酸 (PFOS) 的结构异构体.
结论:
- 开发的MALDI-TOF-MS/TIMS方法为PFAS分析提供了快速,定量和敏感的方法.
- 这种技术促进了PFAS的高通量和现场分析,包括MS成像的潜力.
- 分离异构体的能力为PFAS污染和毒理学提供了更深入的见解.
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