STK19是一种DNA/RNA结合蛋白,对DNA损伤修复和细胞增殖至关重要
Yuling Li1,2, Yanqiu Gong3, Yue Zhou4
1Department of Pulmonary and Critical Care Medicine, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China.
The Journal of cell biology
|January 22, 2024
概括
STK19,现在的TWH19,不是一种酶,而是DNA/RNA结合蛋白,对于DNA修复和细胞增殖至关重要. 它的翅膀螺旋域调解DNA结合,核定位和与修复因子的相互作用.
科学领域:
- 分子生物学分子生物学
- 生物化学 生物化学
- 遗传学 遗传学 是一个
背景情况:
- 此前,STK19已被确定为一种氨酸/氨酸特异性蛋白激酶.
- 它精确的细胞功能一直是科学界争论的主题.
研究的目的:
- 阐明STK19.9的结构和功能特性.
- 澄清STK19在细胞过程中的作用,特别是DNA修复和增殖.
主要方法:
- 采用X射线晶体学来确定STK19.9的三维结构.
- 进行了功能性测试,以评估STK19的DNA/RNA结合能力及其在DNA损伤修复途径中的作用.
- 细胞增殖研究使用STK19敲击模型进行.
主要成果:
- 结构分析显示,STK19缺乏激酶域,而是拥有三个并列的翅膀螺旋 (WH) 域.
- 第三个WH域对同质化,双链DNA结合和核定位至关重要.
- 在核酸切除修复 (NER) 和不匹配修复 (MMR) 中,STK19通过招募RPA2和PCNA等因素来发挥作用.
- STK19结合双链RNA并影响mRNA的表达水平.
- 不管是二元化还是DNA结合突变,STK19的淘汰严重损害了细胞增殖.
结论:
- STK19是一种DNA/RNA结合蛋白,而不是激酶,对于DNA损伤修复 (DDR) 和细胞增殖至关重要.
- 为了避免混,该蛋白被更名为TWH19 (Tandem Winged Helix protein,以前称为STK19),以避免混.
- TWH19在维持基因组稳定性和支持细胞生长方面发挥着至关重要的作用.
相关概念视频
DNA Damage can Stall the Cell Cycle
9.2K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.2K
Single-Strand DNA Binding Proteins
14.1K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
14.1K
Negative Regulator Molecules
35.4K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
35.4K
Nucleotide Excision Repair
3.5K
DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
3.5K
Translesion DNA Polymerases
10.0K
Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
10.0K
Overview of DNA Repair
31.0K
In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
Chemically...
31.0K


