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细胞与细胞的沟通和最初的种群构成塑造了土豆的结构 管 病毒类 准物种
Jian Wu1,2,3, David M Bisaro3
1State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agroproducts, Institute of Plant Virology, Ningbo University, Ningbo 315211, China.
The Plant cell
|January 22, 2024
概括
病毒复制产生了多样化的变体. 马螺杆病毒 (PSTVd) 变体的积累在孤立的细胞中增加,并取决于初始的RNA比率,突出显示细胞通信.
科学领域:
- 植物病毒学 植物病毒学
- 分子进化的分子进化.
- 遗传学 是一个遗传学.
背景情况:
- RNA病毒和 viroid 呈现出高突变率,创造了准物种种群.
- 驱动类比物种动态的机制尚未完全理解.
- 等离子体在 viroid 运动中的作用表明它可能对准物种产生影响.
研究的目的:
- 调查影响马螺杆病毒 (PSTVd) 准物种形成的因素.
- 确定细胞隔离和初始种群组成对PSTVd变种积累的影响.
主要方法:
- 从番茄护卫细胞和培养的中粒细胞原体中测序PSTVd-中间体 (PSTVd-I) 的后代.
- 使用单细胞测序和共感染实验.
- 在不同的感染场景中分析PSTVd-I和变异RNA比率.
主要成果:
- 与整个叶子相比,在孤立的护卫细胞和培养的原生体中观察到更大的变异积累.
- 同感染实验表明,最初的RNA比率显著影响了PSTVd-I的主导地位和变异比例.
- 单细胞感染显示了单细胞内相同或不同的PSTVd序列的多种感染.
结论:
- 细胞间的沟通,特别是缺少等离子体,以及最初的种群组成,对于塑造PSTVd准物种至关重要.
- 这些发现提供了对宿主细胞内RNA病毒和病毒的进化动态的洞察.
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