Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA-seq03:21

RNA-seq

10.0K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.0K
RNA Editing02:23

RNA Editing

9.0K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Response of an Infant With Presumed Multiple Acyl-CoA Dehydrogenase Deficiency (MADD) to Ketone Supplementation.

American journal of medical genetics. Part A·2026
Same author

Prolonged Wnt3a exposure tolerizes macrophages to inflammatory stimuli.

Frontiers in immunology·2026
Same author

The effects and outcomes of the occupational medicine and rehabilitation program for occupational injury subjects - the preliminary results of the One-Stop to Help All-labors (OSHA) Program.

BMC public health·2026
Same author

PFAS Alter Thyroid Histology and Cellular Signaling <i>In Vitro</i> and <i>In Vivo</i>.

Journal of the Endocrine Society·2026
Same author

RAS/MEK/PI3K pathway inhibition augments response to CD40 agonism by targeting CD11b<sup>+</sup> Bregs thereby overcoming melanoma PD1-resistance.

Nature communications·2026
Same author

Microenvironment-aware spatial modeling for accurate inference of cell identity.

Nucleic acids research·2026

相关实验视频

Updated: Jul 5, 2025

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms
05:12

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms

Published on: February 2, 2024

760

FindAdapt:一个python包,用于在小RNA测序中快速准确地检测适配器.

Hua-Chang Chen1,2, Jing Wang1,2, Yu Shyr1,2

  • 1Department of Biostatistics, Vanderbilt University Medical Center, Nashville, Tennessee, United States of America.

PLoS computational biology
|January 22, 2024
PubMed
概括

在没有事先信息的情况下,FindAdapt可以准确地检测小RNA测序数据中的适配器序列. 这个Python包通过简化适配器修剪来提高测序数据集的可重复性和可重复使用性.

更多相关视频

Improving Small RNA-seq: Less Bias and Better Detection of 2'-O-Methyl RNAs
08:49

Improving Small RNA-seq: Less Bias and Better Detection of 2'-O-Methyl RNAs

Published on: September 16, 2019

7.7K
Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

5.6K

相关实验视频

Last Updated: Jul 5, 2025

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms
05:12

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms

Published on: February 2, 2024

760
Improving Small RNA-seq: Less Bias and Better Detection of 2'-O-Methyl RNAs
08:49

Improving Small RNA-seq: Less Bias and Better Detection of 2'-O-Methyl RNAs

Published on: September 16, 2019

7.7K
Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

5.6K

科学领域:

  • 生物信息学是一种生物信息学.
  • 计算生物学 计算生物学
  • 基因组学就是基因组学.

背景情况:

  • 适配器修剪对于小RNA测序 (sRNA-seq) 数据分析至关重要.
  • 现有的工具需要适配器序列,这些序列在公共数据集中经常缺失,不正确或无法访问.
  • 手动的适配器识别是繁的,容易出现错误,特别是随机的适配器.

研究的目的:

  • 开发一种方法,以快速准确地检测sRNA-seq数据中的适配器模式.
  • 提供一个不需要先前适配器序列信息的工具.
  • 提高sRNA-seq数据集的可复制性和可重复使用性.

主要方法:

  • 开发FindAdapt,这是一个用于自动化适配器序列检测的Python包.
  • 使用180个模拟数据集进行评估,这些数据集具有不同的读取结构.
  • 验证了来自各种图书馆准备套件的3184个现实世界sRNA-seq数据集.

主要成果:

  • 在所有测试的模拟数据集中,FindAdapt成功识别了适配器序列.
  • 该工具在大量现实数据集上显示出高准确度.
  • FindAdapt显著优于现有的适配器检测方法.

结论:

  • FindAdapt提供了一个强大的,无信息的解决方案,用于在sRNA-seq.q.中修剪适配器.
  • 该软件可以很容易地集成到现有的生物信息管道中.
  • FindAdapt提高了sRNA-seq数据分析的可靠性和可访问性.