氨基酸和蛋白质的特异性蛋白质脂肪化在C. 伊莱根斯 (elegans) 是一个词
Bingsen Zhang1,2, Yan Yu1,2, Bennett W Fox1,2
1Boyce Thompson Institute, Cornell University, Ithaca, NY 14853.
概括
蛋白质脂化模式因C. elegans中的氨基酸残留而有所不同. 半氨酸主要与分支链脂肪酸S-化,而N-终端甘氨酸使用直链米里斯酸,揭示了残留特异性脂质代谢的整合.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 脂质代谢 脂质代谢是什么
背景情况:
- 蛋白质脂化对于蛋白质的功能和局部化至关重要.
- 脂肪乙基在蛋白质乙化中的利用的多样性和特异性仍然不太清楚.
- 关于结构和起源的S-,N-和O-脂肪化之间的差异尚不清楚.
研究的目的:
- 用非向方法研究脂肪基在蛋白质脂化中的化学多样性和特异性.
- 为了确定S-乙部分结构和起源与N-和O-脂肪乙化有多大不同.
- 为了描述Caenorhabditis elegans中的残留特异性脂肪化模式.
主要方法:
- 利用基胺捕获来识别S-化蛋白.
- 采用酶性蛋白质水解来分析N-和O-化蛋白质.
- 开发了能够进行点击化学的基因探针,用于分支和直链脂肪酸,用于全球分析.
- 进行了同质性搜索,以评估化位图案的保存.
主要成果:
- 在C. elegans中确定了氨酸,N端甘氨酸,氨酸和氨酸残留物中的明显的脂肪化模式.
- 半氨酸主要通过与15-甲基六甲基酸 (isoC17:0) 进行S-化,这是一种单甲基分支链脂肪酸 (mmBCFA).
- N终端甘氨酸主要与直链米里斯酸,氨酸与mmBCFA,氨酸与包括eicosapentaenoic acid在内的各种脂肪酸进行乙基化.
- 全球分析显示了1013个S-化和510个N-或O-化蛋白质,有证据表明蛋白质水平的化特异性.
- 同源性搜索表明,在甲基动物中,化部位模式的高度保存.
结论:
- 蛋白质脂肪化模式是残留物和蛋白质特异性的,整合了脂质代谢的不同分支.
- 这项研究为在氨基酸和蛋白质水平上对蛋白质脂化进行机制研究提供了基础.
- 确定了特定的脂肪酸及其与不同氨基酸残留物的联系,为脂质代谢调节提供了洞察力.
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