维泰林/维泰罗基宁是德国的重要过敏原
Christian Hadiwinarta1, Alex Blank1, Joshua Somers1
1Division of Bacterial, Parasitic, and Allergenic Products, Office of Vaccines Research and Review, Center for Biologics Evaluation and Research, US Food and Drug Administration, Silver Spring, Maryland, USA.
International archives of allergy and immunology
|January 22, 2024
概括
德国 (GCr) 的过敏原,维特洛根因 (Vg) 和维特林 (Vn) 经过净化,被证明是过敏个体IgE抗体的主要标. 这一发现有助于我们更好地理解GCr是如何触发过敏和喘的原因.
科学领域:
- 过敏原研究研究
- 免疫学 免疫学 免疫学
- 昆虫学 昆虫学是一门学科.
背景情况:
- 德国 (GCr) 的空气过敏原与过敏性鼻炎和喘有关.
- 维原蛋白 (Vg) 和维素 (Vn) 是丰富的GCr蛋白和潜在的高分子质量过敏原.
- 之前的净化尝试给出了研究不足的数量.
研究的目的:
- 为了从GCr.中以亲和力净化Vg/Vn.
- 为了确定纯化的Vg/Vn与来自GCr过敏个体的IgE的结合.
主要方法:
- 使用子抗Vg/VnIgG与Sepharose结合的Vg/Vn的亲和性净化.
- 通过SDS-PAGE和LC-HRMS进行纯度和同一性的分析.
- 通过抑制试验评估IgE结合和直接结合到固定Vg/Vn.
主要成果:
- 亲属性染色学产生了91%的纯Vg/Vn.
- 在80%的测试血清中,纯化Vg/Vn抑制了IgE与GCr过敏原的结合.
- 直接IgE结合试验显示,来自GCr过敏个体的80%血清中存在抗Vg/Vn IgE.
结论:
- Vg/Vn 通过亲和染色学成功净化.
- 特定于Vg/Vn的IgE抗体构成了对GCr的整体IgE反应的很大一部分.
相关概念视频
00:42Cross-reactivity
31.1K
Overview
31.1K
01:26Antigens Involved in Adaptive Immunity
497
An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and...
Complete Antigens
Complete antigens possess both immunogenicity and...
497
02:06Allergic Reactions
27.4K
Overview
27.4K
01:10Antibody Structure
60.2K
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
60.2K