混合原体和Geobacter metallireducens之间的电子缩促进了暗发酵:澄清电活性细胞外聚合物质的作用
Hui Li1, Jun Cheng2, Rongxin Xia3
1College of Pipeline and Civil Engineering, China University of Petroleum (East China), Qingdao 266580, China; State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
Bioresource technology
|January 22, 2024
概括
引入Geobacter金属降解剂 (GM) 在黑暗发酵中提高了65.2%的生产. 这就是为什么GMGMGMGMGM
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 环境科学 环境科学
背景情况:
- 通过暗发酵生产的效率受到电子转移效率的限制.
- 优化微生物联盟对于提高生物产量至关重要.
研究的目的:
- 通过使用Geobacter metallireducens (GM) 调节电子转移来改善的生产.
- 调查转基因作为生产污泥 (HPS) 中的电子捐赠者和氧化还原调节器的作用.
主要方法:
- 在黑暗发酵系统中与HPS共同培养转基因.
- 分析产量,微生物群落结构和代谢参数.
- 研究细胞外聚合物质 (EPS) 和c型细胞染色体的作用.
主要成果:
- 最高的产量为306.5mL/g-COD,与单独的HPS相比增加了65.2%.
- 添加转基因改善了NADH/NAD+比率,电子运输系统活性和酶活性.
- 转基因的电活性EPS和与Clostridium sensu stricto的合成相互作用促进了的产生.
结论:
- 在黑暗发酵中,Geobacter metallireducens显著增强了生物的产生.
- 电活性EPS和合成相互作用是改善产量的关键机制.
- 这项研究为生物能源应用提供了对外电素-微生物相互作用的见解.
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