一个多功能肠上芯片系统,用于解读炎症性肠病的免疫病原发生
Oanh T P Nguyen1, Patrick M Misun1, Andreas Hierlemann1
1Bio Engineering Laboratory, Department of Biosystems Science and Engineering, ETH Zurich, Klingelbergstrasse 48, Basel, CH-4056, Switzerland.
Advanced healthcare materials
|January 22, 2024
概括
一个新的肠上芯片模型重建了炎症性肠病 (IBD) 的微环境,使免疫反应的详细研究和对抗炎药物如Infliximab的测试成为可能.
科学领域:
- 生物技术是生物技术.
- 免疫学 免疫学 免疫学
- 胃肠病学 胃肠病学
背景情况:
- 炎症性肠病 (IBD) 的发病过程复杂,需要先进的体外模型.
- 现有的模型往往缺乏人类肠道复杂的细胞和矩阵组成部分.
研究的目的:
- 开发一种新的肠上芯片系统,用于研究IBD免疫病原发生.
- 调查细胞和非细胞成分在IBD期间肠道微环境中的作用.
- 在生理学上相关的IBD模型中评估治疗干预措施.
主要方法:
- 在一周内在亚皮质基质上构建了两极化的肠上皮屏障模型.
- 嵌入了由细胞及其分泌体进行的动态矩阵重塑.
- 将各种免疫细胞和炎症刺激引入到芯片内的特定位置.
- 量化炎症标志物和细胞因子 (TNF-α) 表达.
主要成果:
- 肠上芯片系统成功模拟了肠道微环境和屏障功能.
- 动态矩阵改造证实了芯片上模型的生理相关性.
- 经过刺激时,已经显示出高调的炎症标志物和TNF-α表达.
- 展示了Infliximab在中和TNF-α和相关细胞因子方面的有效性.
结论:
- 开发的微生理系统为了解IBD免疫病原发生提供了一个强大的工具.
- 这种模型有助于系统地调查免疫媒介肠道疾病.
- 该系统有可能识别和验证IBD的新治疗策略.
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