血红蛋白E标志的不常见原因在通过离子交换高性能液态染色学测量血红蛋白A1c时被确定
Thomas Herb1, Alexander S Taylor1, Shih-Hon Li1
1Department of Pathology, University of Michigan, Ann Arbor, MI, US.
Laboratory medicine
|January 22, 2024
概括
不一致的血红蛋白A1c (HbA1c) 查结果,标记为怀疑的血红蛋白E (HbE),可能来自罕见的变体,如Hb Hoshida,真实的HbE或分析文物. 适当的实验室工作对于准确的诊断和患者护理至关重要.
科学领域:
- 临床化学 临床化学
- 血液学 血液学 血液学
- 分析生物化学 分析生物化学
背景情况:
- 血红蛋白A1c (HbA1c) 是糖尿病诊断和管理的关键生物标志物.
- 离子交换高性能液态染色学 (HPLC) 是用于测量HbA1c的常用方法.
- 某些血红蛋白变异可以干扰HbA1c测定,导致不准确的结果.
研究的目的:
- 呈现和分析三例不一致的HbA1c结果,标记为疑似血红蛋白E (HbE).
- 调查HPLC测量HbA1c干扰的各种原因.
- 提出一种实验室模型,用于调查和报告由于血红蛋白变异引起的不一致的HbA1c结果.
主要方法:
- 分析了三名患者的病例研究,这些患者的HbA1c查结果不一致.
- 仪器标记"怀疑的血红蛋白E (HbE) "作为进一步调查的触发.
- 实验室工作,包括血红蛋白变体识别 (例如,Hb Hoshida,HbE) 和分析文物评估.
主要成果:
- 案例1:由于一种罕见的血红蛋白变体引起的异调HbA1c,被确定为血红蛋白Hoshida.
- 案例2:由于真异合的HbE,导致异调的HbA1c.
- 案例3:由分析工件引起的不一致的HbA1c导致HbA1c峰值分裂而没有底层变异.
结论:
- "疑似HbE"标志表明HbA1c测量中存在广泛的潜在干扰.
- 系统的实验室方法对于分类干扰和确保准确的HbA1c报告至关重要.
- 需要在HbA1c测定中对血红蛋白变异干扰进行标准化的报告协议.
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