生殖组织中的分子定量特征位点对牛的雄性生育能力产生影响
Xena Marie Mapel1, Naveen Kumar Kadri1, Alexander S Leonard1
1Animal Genomics, ETH Zurich, Universitatstrasse 2, 8092, Zurich, Switzerland.
Nature communications
|January 22, 2024
概括
这项研究分析了公牛的转录组,以揭示影响男性生育能力的遗传因素. 研究人员确定了关键的基因和与牛繁殖成功相关的分子变异.
科学领域:
- 基因组学就是基因组学.
- 生殖生物学 生殖生物学
- 动物科学动物科学
背景情况:
- 繁殖公牛是研究雄性生育能力的有价值模型,因为它们具有广泛的基因型和生殖数据.
- 来自牛生殖组织的功能性基因组数据有限,这阻碍了与生育相关区域的精细绘制.
研究的目的:
- 鉴定公牛生殖组织 (丸,上皮,分泌管) 中的基因表达和拼接变异的特征.
- 识别与分子表型和全基因组变异相关的调节位置.
- 发现与男性生育能力的定量变异相关的基因和分子表型.
主要方法:
- 从118只成熟的公牛身上,测序了丸,脊髓和分泌管的转录组序列.
- 分子表型与全基因组变异之间的关联测试.
- 转录组范围的关联和局部化分析.
主要成果:
- 在三种牛生殖组织中特征基因表达和拼接变异.
- 通过将分子表型与全基因组变异联系起来,确定了41,156个调节位点.
- 在牛,人类和小鼠生殖组织之间展示了保存的基因表达模式.
- 发现表达和拼接介导的变体在丸中明显更普遍.
- 确定了与男性生育能力变异相关的特定基因 (WDR19,SPATA16,KCTD19,ZDHHC1).
结论:
- 这项研究为牛生殖组织提供了全面的功能基因组数据,增强了我们对男性生育能力的理解.
- 试管体表现出较高的转录复杂性,而不是 epididymis 和 deferens 管.
- 确定了在牛养殖计划中改善男性生育能力的新型遗传和分子标.
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