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一种基于转子子的基因标记物,用于在Bactrocera dorsalis物种复合体内的同种特征

Grazyna J Zimowska^1,2, Nirmala Xavier^1,2, Masroor Qadri^1,2

¹U.S. Department of Agriculture, Center for Medical, Agricultural, and Veterinary Entomology, Agricultural Research Service, 1700 SW 23rd Drive, Gainesville, FL, 32608, USA.

Scientific reports

|January 22, 2024

概括

研究人员确定了突变的piggyBac转位子序列及其插入部位作为可靠的分子标记物,用于识别Bactrocera dorsalis复合体内的物种. 这种方法可以有效地区分密切相关的物种和菌株.

科学领域:

基因组学就是基因组学.
分子生物学分子生物学
昆虫学昆虫学是一门学科.

背景情况:

准确的物种识别在分类学和害虫管理中至关重要.
可转换的元素,如piggyBac,进化,可以显示序列变化.
背脊菌 (Bactrocera dorsalis) 种群复杂存在分类学上的挑战,因为它们的相似性很高.

研究的目的:

开发一种分子方法来评估同类型的身份.
为了研究突变的piggyBac转位子序列作为物种标记物的实用性.
为了区分在Bactrocera dorsalis复合体内密切相关的物种.

主要方法:

从B.dorsalis.中分离和测序一个突变的piggyBac转位子 (pBacBd-Kah).
使用PCR与向pBacBd-Kah序列和相邻的基因组DNA的原始体.
对不同Bactrocera物种和菌株的同源序列和插入部位进行比较分析.

主要成果:

在B. papayae,B. philippinensis和B. invadens中发现了相同的pBacBd-Kah同源元素和插入点,支持它们与B. dorsalis. 的同义词.
B. carambolae表现出pBacBd-Kah序列/插入部位 (苏里南菌株) 和一个分离的衍生物 (法属圭亚那,孟加拉国,马来西亚菌株).
在更遥远的相关的Bactrocera物种中,没有pBacBd-Kah元素.

结论:

突变的piggyBac转位子序列及其特定的基因组插入点作为同类型身份的可靠标记.
这种分子方法有效地区分了与Bactrocera属内的密切相关的物种和菌株.
侵入性移动元素为分子物种识别提供了强大的工具.

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