转录启动方向性的差异是基因和cis-regulatory元素的染色质修饰模式中植物和动物之间的区别的基础
Brianna D Silver1,2, Courtney G Willett1,2, Kelsey A Maher1,3,4
1Department of Biology, Emory University, Atlanta, GA 30322, USA.
G3 (Bethesda, Md.)
|January 22, 2024
概括
植物基因组显示单向转录启动,不同于动物基因组显示双向启动在促进器和cis-regulatory元素. 这种根本的差异影响了RNA聚合酶启动部位附近的染色质修饰.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 细胞基因表达在转录启动时受到调节,这是由RNA聚合酶II控制的关键步骤.
- RNA聚合酶II经常在动物和真菌中的促进体和cis-regulatory元素中双向启动转录,但通常在基因中单向延长.
- 之前在植物 (如Arabidopsis thaliana) 上的研究表明,上游转录启动有限.
研究的目的:
- 在植物和动物基因组中比较转录相关的质子修饰和转录起点的新生转录和 cis-regulatory 元素.
- 为了研究RNA聚合酶II启动在Arabidopsis thaliana,Drosophila melanogaster和Homo sapiens中的方向性.
- 为了确定单向转录启动是否保留在其他植物基因组,如大米和大豆.
主要方法:
- 对转录相关的基因组修饰的比较分析.
- 使用高通量方法对新生的转录进行分析.
- 检查RNA聚合酶II启动模式在促进体和cis-regulatory元素.
主要成果:
- 阿拉比多普西斯塔利亚纳在促进体和基因近邻的cis-regulatory元素中表现出主要的单向RNA聚合酶II启动.
- 双向转录启动在Drosophila melanogaster和Homo sapiens中的促进体和Drosophila中的cis-regulatory元素中广泛存在.
- 对大米和大豆基因组的分析表明,单向转录启动是常态.
结论:
- 开花植物和元动物基因组之间的转录启动方向性存在根本的差异.
- 这些差异体现在RNA聚合酶启动部位周围不同的染色质修饰模式中.
- 单向启动似乎是植物基因组中保存的特征,与动物的双向启动形成鲜明对比.
关键词:
这是一个 cis-regulatory 元素.这种RNA聚合酶是RNA聚合酶.增强剂是一种增强剂.基斯顿基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因活动主办人 活动主办人转录 转录 是一种转录.更多相关视频
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