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Updated: Jun 26, 2026

Bioluminescent Bacterial Imaging In Vivo
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在真核生物中进行自主生物发光成像的改进途径

Ekaterina S Shakhova1,2, Tatiana A Karataeva1,2, Nadezhda M Markina1,2

  • 1Planta LLC, Moscow, Russia.

Nature methods
|January 22, 2024
PubMed
概括

研究人员优化了真菌生物发光通路,以在植物,真菌和动物中获得更明亮的光线. 这一突破使各种宿主生物体的成像能力得到增强.

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Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
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Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.