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在羊中发现具有小DNA基因组的新型真核病毒的元基因组识别
Eszter Kaszab1,2,3, Krisztina Bali1,2, Szilvia Marton1,2
1HUN-REN Veterinary Medical Research Institute, 1143 Budapest, Hungary.
Animals : an open access journal from MDPI
|January 23, 2024
概括
对羊肠道样本的基因组分析揭示了各种RNA和DNA病毒,包括新型帕沃病毒和循环Rep编码单链DNA病毒. 这一发现有助于开发诊断工具并了解它们对动物健康的影响.
科学领域:
- 兽医病毒学 兽医病毒学
- 转基因组学是指转基因组学.
- 鸟类健康 鸟类健康
背景情况:
- 肠道病毒在禽类的健康和疾病中起着重要作用.
- 超基因组方法为在野生动物群体中发现新型病毒病原体提供了强大的工具.
研究的目的:
- 使用元基因组学分析,研究普通 (Phasianus colchicus) 的肠道中的病毒多样性.
- 为了识别和表征新型RNA和DNA病毒存在于鹿肠道样本.
主要方法:
- 不偏向的丰富协议和下一代测序被用于病毒DNA和RNA提取.
- 生物信息管道被用于分析序列读取和识别病毒基因组.
- 对已识别的病毒进行了基因组重建.
主要成果:
- 检测到广泛的真核病毒,包括冠状病毒,reoviruses,parvoviruses和循环Rep编码单链DNA (CRESS DNA) 病毒.
- 对多种帕尔沃病毒 (dependoparvovirus,aveparvovirus,chaphamaparvovirus),陀螺病毒和CRESS DNA病毒进行了部分或完整的基因组序列重建.
- 病毒序列读取率在样本之间有显著差异,从0.01%到59%.
结论:
- 这项研究在普通羊中发现了以前未知的丰富多样性的病毒.
- 这些病毒多样性数据为开发有针对性的诊断工具和流行病学研究提供了基础.
- 了解这些新型病毒对于评估它们对羊健康和福利的影响至关重要.
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