来自L.Ficus carica的一种多糖体,在体外和体外实验模型中都具有免疫调节活性
Lin Ye1, Qin-Qiu Zhang1, Shang Lin1
1College of Food Science, Sichuan Agricultural University, Ya'an 625014, China.
Foods (Basel, Switzerland)
|January 23, 2024
概括
来自Ficus carica L.的无花果多糖 (FCP) 被净化并进行了表征. 在免疫抑制小鼠中,FCP通过刺激巨细胞活动和增加促炎因素来表现出免疫调节作用.
科学领域:
- 自然产品化学 自然产品化学
- 免疫学 免疫学 免疫学
- 药理学 药理学是指药理学的学科.
背景情况:
- 来自Ficus carica L. (FCP) 的多糖具有多样化的生物活动.
- 关于纯化"布朗斯维克"无花果多糖的特定结构和机制的信息有限.
- 了解FCP的特性对于其在与健康相关的行业中的应用至关重要.
研究的目的:
- 从"布伦瑞克无花果"中化学净化和表征FCP.
- 调查FCP的体外和体外免疫调节作用.
- 为了阐明FCP诱导的巨细胞激活的机制.
主要方法:
- 化学净化和FCP的仪器分析.
- 在体外测试测量氧化 (NO),TNF-α和IL-6的产生,以及巨细胞的细胞活性.
- 使用免疫抑制小鼠进行体内研究,以评估FCP对促炎因素的影响.
- 分析FCP与托尔类受体4 (TLR4) 的相互作用.
主要成果:
- 纯化FCP的分子量为127.5kDa,主要由银河酸,阿拉比诺斯和银河糖组成.
- 在实验室中,FCP刺激了NO,TNF-α和IL-6的产生,并增强了巨细胞化.
- 通过与托尔类受体4 (TLR4) 的相互作用,FCP激活了巨细胞.
- 在体内,FCP显著增加了免疫抑制小鼠的血清促炎因素.
结论:
- FCP表现出显著的免疫调节特性.
- FCP通过TLR4信号来激活巨细胞.
- FCP有可能成为用于制药和功能食品的新型免疫调节剂.
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