植物病毒编码的蛋白质的预测建模为它们的结构和固有的多功能性提供了新的了解
Brandon G Roy1, Jiyeong Choi1, Marc F Fuchs1
1Plant Pathology and Plant-Microbe Biology Section, School of Integrative Plant Science, Cornell University, 15 Castle Creek Drive, Geneva, NY 14456, USA.
Biomolecules
|January 23, 2024
概括
预测建模揭示了葡萄藤扇叶病毒 (GFLV) 蛋白质的新功能,增强了我们对病毒蛋白质作用和结构的理解. 这项研究为GFLV蛋白功能及其卫星蛋白提供了新的见解.
科学领域:
- 植物病毒学 植物病毒学
- 分子生物学分子生物学
- 生物信息学是一种生物信息学.
背景情况:
- 植物病毒编码为其生命周期所必需的多功能蛋白质.
- 许多病毒蛋白的完整功能表仍然不完全理解.
- 预测建模提供了一种强大的方法来阐明蛋白质功能.
研究的目的:
- 通过计算预测和验证由葡萄藤扇叶病毒 (GFLV) 基因组RNA及其卫星RNA编码的蛋白质的功能.
- 为了确定GFLV蛋白质组内的新型蛋白质功能和域.
- 为了提高对GFLV蛋白质结构-功能关系的理解.
主要方法:
- 利用一套蛋白质预测软件来分析GFLV RNA1,RNA2和卫星RNA编码的蛋白质.
- 确认了先前验证的和假定的蛋白质功能,包括RNA沉默抑制剂 (VSR),病毒基因组链接蛋白 (VPg),蛋白酶 (Pro) 和运动蛋白 (MP).
- 预测了新的蛋白质域和功能,如T3 / T7样RNA聚合酶,短链还原酶,ABC载体和DNA拓酶域,具有不同的置信度.
主要成果:
- 已确认的已知功能 (VSR, VPg, Pro, MP, CP, Td, Sd, HP) 和假定的功能 (Hel, Pol) 的GFLV蛋白质.
- 确定了蛋白质1A,1B,1E,2B和卫星RNA蛋白的新域和潜在功能.
- 结构预测显示出不同的信心,对蛋白质1A,1B,1C,1D,1E和2C的信心更高.
结论:
- 预测建模成功扩展了已知的GFLV蛋白及其卫星蛋白的功能表.
- 这项研究为GFLV编码的蛋白质的结构和功能多样性提供了新的见解.
- 需要进一步的实验验证,以确认预测的新功能.
相关概念视频
00:56Viral Structure
62.3K
Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.
62.3K
02:41Protein Complex Assembly
10.6K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types. Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Many viruses self-assemble into a fully functional unit using the infected host cell to...
10.6K
01:56Structural Protein Function
27.7K
Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to...
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to...
27.7K
02:28Leaky Scanning
5.1K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.1K
01:25Cell Signaling in Plants
5.6K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.6K
02:22Introduction to Plant Diversity
44.7K
From Water to Land
44.7K