在洛卡特水果发育中的SBP-Box基因家族的全基因组识别和表达分析
Haiyan Song1,2,3, Ke Zhao1,2, Guoliang Jiang1,2
1Horticulture Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China.
Genes
|January 23, 2024
概括
研究人员在白鱼中发现了28个SBP-box基因,发现了两个关键基因EjSBP01和EjSBP19,可能调节白鱼水果发育中的胡卜素合成和水果成熟.
科学领域:
- 植物遗传学和分子生物学
- 果子的发育和成熟.
- 植物中的营养生物合成.
背景情况:
- 洛卡特 (Eriobotrya japonica) 果实因其药用特性和诸如胡卜素之类的营养素而受到重视.
- SBP-box基因对植物生长至关重要,但它们在瓜水果发育中的作用尚不清楚.
- 了解瓜果的成熟和胡卜素积累需要识别相关的基因.
研究的目的:
- 为了识别和表征SBP-box基因家族在loquat基因组.
- 为了研究水果发育过程中SBP-box基因的表达模式.
- 为了确定参与卡洛类生物合成和果实成熟的候选SBP-box基因.
主要方法:
- 在loquat.at中对28个EjSBP基因进行全基因组识别.
- 对EjSBP蛋白质的遗传学分析,基因结构,保存型号和cis元素分析.
- 定量实时PCR (qRT-PCR) 分析不同组织和水果类型的基因表达 (白色与黄色肉质).
主要成果:
- 二十八个EjSBP基因被确定并基于基因组分布,系系和结构进行了表征.
- 大多数EjSBP基因在植物组织中高度表达,其中五个显示出高果实表达.
- 白色和黄色果实之间有8个EjSBP基因的差异表达,EjSBP01和EjSBP19显示了与胡卜素积累相关的独特模式.
结论:
- 鱼中的SBP盒基因家族已被全面识别和表征.
- EjSBP基因在瓜水果的发育中起着重要作用,特别是在破碎阶段.
- EjSBP01和EjSBP19是参与调节类化合物合成和积累在果成熟期间的强有力的候选者.
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