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衰老和高脂肪饮食对脂肪组织衍生的细胞外囊 miRNA 形状的影响

Young-Eun Cho¹, Shaoshuai Chen¹, Keith Crouch¹

¹College of Nursing, The University of Iowa, 50 Newton Road, Iowa City, IA 52242, USA.

|January 23, 2024

概括

衰老和高脂肪饮食会改变脂肪组织细胞外囊泡 (EV) 中的微RNA特征. 这些EV微RNA可能在与年龄和肥胖相关的疾病中发挥作用.

科学领域:

代谢性疾病研究研究.
老龄化和长寿研究研究.
细胞外囊泡的分子生物学

背景情况:

中年成人面临高肥胖率,导致严重的健康问题.
肥胖来自多余的脂肪组织 (AT) 释放改变的细胞外囊泡 (EVs).
这些电动汽车有助于各种与年龄有关的健康并发症.

研究的目的:

为了研究年龄和高脂肪饮食对AT衍生的EV小RNA配置文件的影响.
了解这些miRNAs在衰老和肥胖中的作用.
为了识别受饮食和衰老影响的特定miRNA.

主要方法:

在年轻和中年群体中,小鼠被食正常的或高脂肪饮食.
评估了代谢特征.
脂肪组织的细胞外囊泡 (EVs) 被分离并对miRNA含量进行分析 (n=599).

主要成果:

中年老鼠无论饮食如何都会肥胖; HFD 的年轻老鼠模仿了这一点.
在中年小鼠和正常饮食的年轻小鼠中发现了131种差异表达的miRNA (DE-miRNA).
55个DE-miRNA在中年老鼠和年轻的高脂肪饮食中的小鼠中被确定,与抑制IGF1R活动有关.

结论:

脂肪组织 (AT) EV miRNAs显著影响与衰老和肥胖相关的疾病.
特定的miRNA如miR-21,miR-148a和miR-29a都与此有关.
EV miRNA 分析提供了对代谢和衰老疾病机制的见解.

关键词:

脂肪组织的脂肪组织.老化的老化衰老的老化细胞外囊泡中的细胞外囊泡.这就是miRNAs.肥胖肥胖肥胖肥胖肥胖肥胖肥胖肥胖

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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...