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相关概念视频

CRISPR01:59

CRISPR

51.1K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Homologous Recombination02:31

Homologous Recombination

50.6K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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相关实验视频

Updated: Jul 5, 2025

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
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A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

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作为一种突变性因子的CRISPR/Cas9.

Andrey R Shumega1, Youri I Pavlov2,3, Angelina V Chirinskaite4

  • 1Department of Genetics and Biotechnology, St. Petersburg State University, 199034 St. Petersburg, Russia.

International journal of molecular sciences
|January 23, 2024
PubMed
概括
此摘要是机器生成的。

克里斯普尔/卡斯9基因编辑功能强大,但可能导致有害突变. 研究人员审查了它的突变性特性和降低风险的方法,以获得更安全的基因组编辑应用.

关键词:
这就是CRISPR/Cas9的作用.基因组编辑 基因组编辑突变是发生在突变中的突变.目标之外的活动活动.

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科学领域:

  • 遗传学 是一个遗传学.
  • 分子生物学分子生物学
  • 生物技术是生物技术.

背景情况:

  • 基因编辑CRISPR/Cas9已经改变了遗传研究和治疗应用.
  • 克里斯普尔/卡斯9系统在目标和非目标部位诱导DNA断裂.
  • 来自DNA修复途径的意外突变可能导致疾病.

研究的目的:

  • 描述CRISPR/Cas9作为一种突变性因子.
  • 讨论CRISPR/Cas9.9的突变性特性.
  • 审查影响CRISPR/Cas9突变性因素.

主要方法:

  • 对CRISPR/Cas9的突变性特性进行审查.
  • 在基因组编辑中分析DNA修复途径.
  • 检查减轻非目标效应的策略.

主要成果:

  • 克里斯普尔/卡斯9作为一种强大的突变原体.
  • 非目标突变和不精确的DNA修复有助于不需要的遗传改变.
  • 修改的Cas9核酶,改进的传递和有针对性的修复途径可以降低风险.

结论:

  • 由于CRISPR/Cas9技术具有突变性潜力,因此需要谨慎管理.
  • 了解和控制CRISPR/Cas9诱导的突变对于安全的基因组编辑至关重要.
  • 未来的研究应该专注于提高基因编辑应用的精度和最小化非目标效应.