相关实验视频

Updated: Jul 5, 2025

Extraction, Labeling, and Purification of Lineage-Specific Cells from Human Antral Follicles

Extraction, Labeling, and Purification of Lineage-Specific Cells from Human Antral Follicles

Published on: November 30, 2022

单细胞转录组分析揭示了在绵羊卵细胞中不同同步的额头毛囊大小的发育特异性网络

Yukun Song¹, Nan Zhang¹, Yu Zhang¹

¹Inner Mongolia Key Laboratory of Sheep & Goat Genetics Breeding and Reproduction, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.

International journal of molecular sciences

|January 23, 2024

概括

这项研究绘制了羊卵细胞转录组在腹膜毛囊发育期间的地图,揭示了特定阶段的基因特征和信号通路,这对于卵细胞成熟和毛囊生长至关重要.

科学领域:

生殖生物学生殖生物学
基因组学就是基因组学.
卵细胞生物学卵细胞生物学

背景情况:

卵细胞发育是一个复杂的,受监管的过程,由卵细胞本身指挥.
了解卵子转录特征是卵子成熟和卵泡发育的关键.
之前的研究描述了体外成熟和排卵前卵子细胞的转录特征,但不描述 antral 毛囊中的转录特征.

研究的目的:

描述羊卵细胞在小,中,大毛囊中的转录组景观.
识别特定阶段的分子特征,卵细胞分泌因子和转录因子网络.
阐明与卵细胞质量和体外成熟能力相关的信号通路.

主要方法:

使用了单细胞转录组学 (SmartSeq2).
从小,中,大腹毛囊中分析了绵羊卵细胞.
转录组数据被用来绘制分子签名和调节网络的地图.

主要成果:

在毛囊发育期间生成了绵羊卵细胞的综合转录图谱.
在大卵细胞 (LO) 中发现了222个基因,在中等卵细胞 (MO) 中发现了8个基因,在小卵细胞 (SO) 中发现了6个基因.
阐明了反映卵细胞质量和体外成熟能力的信号通路.
发现了驱动毛囊招募和选择的关键生物过程和枢纽基因.

结论:

这项研究提供了对绵羊单卵细胞转录组的全面描述,填补了关键的知识空白.
这些发现提供了对控制毛囊发育的转录调节的见解.
了解这些机制对于理解卵子细胞如何协调卵泡发育和质量至关重要.

关键词:

腹毛囊是腹毛囊中的一个.分子签名分子签名羊卵细胞是羊卵细胞.信号通道的信号通道.单细胞转录组学单细胞转录组学

更多相关视频

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

Published on: June 16, 2021

Isolation of Small Preantral Follicles from the Bovine Ovary Using a Combination of Fragmentation, Homogenization, and Serial Filtration

Isolation of Small Preantral Follicles from the Bovine Ovary Using a Combination of Fragmentation, Homogenization, and Serial Filtration

Published on: September 27, 2022

相关概念视频

Oogenesis

Oogenesis

In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...

Folliculogenesis

Folliculogenesis

Folliculogenesis is the development of ovarian follicles, the specialized structures within the ovarian cortex where oogenesis, or egg development, occurs. This process is essential for female reproductive health and begins during fetal development when primordial follicles are formed. Each primordial follicle comprises a primary oocyte in the center, surrounded by a single layer of squamous pre-granulosa cells. These follicles remain dormant in late prophase I of meiosis until triggered by...