用生物技术方法制备的家禽凝的特性,以在分子水平上进行有针对性的变化
Aneta Prokopová1, Pavel Mokrejš1, Robert Gál2
1Department of Polymer Engineering, Faculty of Technology, Tomas Bata University in Zlín, 760 01 Zlín, Czech Republic.
International journal of molecular sciences
|January 23, 2024
概括
肉凝为高级应用提供了一个有前途的来源. 这项研究证实了其抗氧化特性,适当的分子量分布和微生物安全性,使其成为传统动物衍生原蛋白的可行替代品.
科学领域:
- 生物技术和生物材料科学 生物技术和生物材料科学
- 食品科学与技术 食品科学与技术
- 生物化学 生物化学
背景情况:
- 肉原蛋白是一种有价值的,未充分利用的来源,用于生产凝和水解剂.
- 用酶进行生物技术加工,可以在分子层面上定制凝的特性.
- 有针对性的凝性质对于先进的化品,制药,医疗和食品应用至关重要.
研究的目的:
- 为了描述肉凝的抗氧化活性,分子重量分布和功能组.
- 评估肉凝在各种应用中的微生物安全性.
- 评估肉凝作为传统原蛋白来源的可持续替代品.
主要方法:
- 使用DPPH (2,2-二-1-皮克里利水) 和ABTS (2,2-阿齐诺比斯-3-乙烯扎林-6-硫酸) 试验评估的抗氧化活性.
- 用凝透色谱与折射计检测 (GPC-RID) 确定的分子重量分布.
- 福里埃变换红外光谱学 (FTIR) 分析了功能组和多链配置.
- 使用MALDI (矩阵辅助激光脱离离电离) 评估的微生物群体包括Sabouraud乳糖 (SDA),盘子计数 (PCA),三性大豆 (TSA) 和紫色红胆汁乳糖 (VRBL).
主要成果:
- 肉凝表现出显著的抗氧化活性,超过ABTS基的80%和DPPH基的69%.
- 在GPC-RID分析中,所有凝样本中都发现了典型的α,β和γ链.
- FTIR光谱学证实了特征性的原分裂产品振动区域的存在 (胺基A,B,I,II,III).
- 微生物学分析表明没有不良的细菌污染,确保适合先进的应用.
结论:
- 肉凝具有显著的抗氧化能力和理想的分子特性.
- 该材料在微生物学上是安全的,支持其在苛刻的应用中使用.
- 肉凝是一种可行的,有前途的替代品,可以替代来自陆地动物来源的凝.
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