对代谢组和转录组的网络分析揭示了不同度的调节在杂交的变形发育过程中
Shuang Zhang1, Lina Cao2, Ruhui Chang1
1College of Life Science, Northeast Forestry University, Harbin 150040, China.
International journal of molecular sciences
|January 23, 2024
概括
的供应会影响杂交变形的发展,在低度下减少细胞层. 这项研究揭示了关键的基因表达和涉及的代谢途径.
科学领域:
- 植物生物学 植物生物学
- 林业科学 林业科学
- 分子生物学分子生物学
背景情况:
- 木质植物的二次发育对于木材形成至关重要.
- 外源会影响二次生长,但其对的影响尚不清楚.
- 了解在木中的作用对于木材开发研究至关重要.
研究的目的:
- 为了研究不同度对杂交变形植物发育的影响.
- 确定气对变异体的影响背后的分子和代谢机制.
- 提供有关调节木材形成的新见解.
主要方法:
- 在不同度 (0.15 mM, 0.3 mM, 1 mM, 3 mM, 5 mM NH4NO3) 下,对杂交木变体的复制体和代谢组分析.
- 差异基因表达 (DEG) 分析以确定受影响的基因.
- 代谢物分析,以检测代谢途径的变化.
- 网络分析以整合转录组和代谢组数据.
主要成果:
- 低 (0.15毫米和0.3毫米NH4NO3) 与1毫米NH4NO3.3相比,更细胞层的减少.
- 高度 (3mM和5mM NH4NO3) 并没有显著改变变化细胞层.
- 在激素信号转导,烯酸生物合成,粉/糖代谢和蛋白质分解途径中显著影响了基因表达.
- 不同的代谢物被富含着黄类,类,氨酸和糖类.
结论:
- 的度会影响杂交变体的发育,低度会减少细胞层.
- 植物激素信号转导,烯胺生物合成和粉/糖代谢途径可能参与的调节.
- 这项研究提供了关于气对木材形成的分子和代谢调节的新见解.
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