运动干预的内皮原生细胞外体通过改善线粒体功能来保护N2a细胞
Shuzhen Chen1, Smara Sigdel1, Harshal Sawant1
1Department of Biomedical Sciences, Joan C. Edwards School of Medicine, Marshall University, Huntington, WV 25755, USA.
International journal of molecular sciences
|January 23, 2024
概括
在高血压中,运动干预增强了内皮原生细胞衍生的外体组 (EPC-EX) 功能. 这些运动修饰的EPC-EX可以改善脑细胞存活率和线粒体功能,可能是通过恢复miR-27a水平.
科学领域:
- 心血管生物学 心血管生物学
- 神经科学是一个神经科学.
- 细胞生物学 细胞生物学
背景情况:
- 高血压会损害内皮原生细胞 (EPC) 和大脑内皮细胞之间的外体通信,可能会恶化中风的结果.
- 运动是一种潜在的干预措施,但其对高血压中EPC-exosome (EPC-EX) 功能的影响尚不清楚.
研究的目的:
- 调查运动干预是否可以在高血压条件下恢复EPC外体 (EPC-EX) 功能.
- 确定miR-27a在EPC-EXs运动中介改善中的作用.
主要方法:
- 从静坐和运动高血压小鼠中生成EPC-EXs.
- 在 angiotensin II (Ang II) 加上缺氧下分析了外体微RNA 概况并评估了 N2a 细胞中的 EPC-EX 功能.
- 评估了EX吸收,细胞存活率,活性氧物种 (ROS),线粒体膜潜力和关键蛋白质表达 (cytochrome c, Nox4).
主要成果:
- 运动改善了N2a细胞的EPC-EX吸收,并恢复了EPC-EXs中的miR-27a水平.
- 运动修饰的EPC-EXs (R-EPC-EXET) 增强了N2a细胞存活率,减少了ROS,并在高血压压力下改善了线粒体功能.
- 这些好处被miR-27a抑制剂显著减少,表明其关键作用.
结论:
- 运动干预可以恢复高血压条件下EPC-EXs的保护功能.
- 运动改变的EPC-EXs,特别是通过它们携带的miR-27a,改善脑细胞线粒体功能和生存.
- 这突显了一种新的治疗途径,涉及外体通信,用于管理与高血压相关的神经风险.
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