相关实验视频

Updated: Jul 5, 2025

Identification of Circular RNAs using RNA Sequencing

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

基于长非编码和循环RNA分析的循环节律的新洞察力

Xiaodong Tan¹, Jiawen Zhang¹, Jie Dong¹

¹Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.

International journal of molecular sciences

|January 23, 2024

概括

这项研究探讨了中昼夜节律的表观遗传调节,确定了关键的非编码RNA和AOX1对于调节光暗循环至关重要,并可能影响人类健康.

科学领域:

动物模型动物模型
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
时间生物学时间生物学

背景情况:

循环节律障碍对人类健康和动物生产构成重大风险.
下丘脑是昼夜节律调节的中心.
农场动物中昼夜节律的表观遗传调节仍未得到充分研究.

研究的目的:

调查中昼夜节律调节的表观遗传机制.
识别与光/黑暗循环相关的新型非编码RNA (ncRNA) 和mRNA生物标记物.
探索ncRNAs在调节昼夜节律和相关代谢途径中的作用.

主要方法:

在24小时的光/黑暗周期中收集体下丘脑样本.
执行了长非编码RNAs (lncRNAs),圆形RNAs (circRNAs) 和信使RNAs (mRNAs) 的全面测序.
利用差异表达和昼夜分析来识别调节网络.

主要成果:

确定了两个与光/黑暗循环相关的基因组 (昼夜节律和视网膜代谢).
发现了具有昼夜表达模式的非编码RNA网络,包括38个lncRNA,15个circRNA和200个候选基因.
通过影响视网膜代谢中的AOX1来确定三个lncRNA和一个circRNA作为昼夜节律的关键调节者.

结论:

这项研究提供了关于使用模型对昼夜节律的表观遗传调节的见解.
确定了参与昼夜节律控制的特定lncRNA和circRNA.
AOX1被强调为对昼夜节律调节和相关疾病的潜在治疗标.

关键词:

一个AOX1AOX1 昼夜节律昼夜节律圆形的RNARNA是一个圆形的RNA.在下丘脑中,下丘脑长非编码RNA是什么意思

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