相关实验视频

Updated: Jul 5, 2025

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

Published on: June 20, 2018

SpliceProt 2.0:人类,老鼠和老鼠蛋白质的序列存储库

Letícia Graziela Costa Santos¹, Vinícius da Silva Coutinho Parreira¹, Esdras Matheus Gomes da Silva^1,2

¹Instituto Carlos Chagas, Fundação Oswaldo Cruz (FIOCRUZ), Rua Professor Algacyr Munhoz Mader 3775, Cidade Industrial De Curitiba, Curitiba 81310-020, PR, Brazil.

International journal of molecular sciences

|January 23, 2024

概括

"SpliceProt 2.0"是一个更新的蛋白质基因组学数据库,可以识别人类,小鼠和老鼠的新蛋白质序列和蛋白质形式. 该资源有助于验证拼接变体并预测跨物种的正统蛋白质.

科学领域:

蛋白质基因组学是什么
生物信息学是一种生物信息学.
计算生物学计算生物学

背景情况:

人类,小鼠和老鼠的蛋白质组含有已知的蛋白质和潜在的新型蛋白质.
现有的数据库可能无法完全捕捉拼接变体,需要先进的蛋白质组验证工具.
了解正体蛋白对于比较基因组学和功能研究至关重要.

研究的目的:

推出SpliceProt 2.0,一个增强的公共蛋白质基因组学数据库.
提供计算翻译蛋白质的全面存储库,包括新型蛋白质形式.
为了促进拼接变体的蛋白质学验证和正统蛋白质的预测.

主要方法:

使用三元矩阵进行转录重建,使用来自Ensembl,UniProt和APPRIS的数据.
集成的转录重建,序列注释,并在转录组和蛋白质组层面检测.
预计被排除的转录会通过NMD路径易受降解.

主要成果:

与之前的版本相比,SpliceProt 2.0提供了更广泛的计算翻译蛋白质.
证明了SpliceProt 2.0在预测人类和小鼠之间的正统蛋白质中的实用性.
在UniProtKB/SwissProt参考数据库中没有成功识别的拼接变体.

结论:

"SpliceProt 2.0"为蛋白质基因组学社区提供了宝贵的资源.
该数据库有助于发现和验证新型蛋白质形式和拼接变体.
它的应用扩展到预测正体蛋白质,增强比较蛋白质组研究.

关键词:

蛋白质基因组学蛋白质组学蛋白质组学转录组信息的蛋白质数据库.

更多相关视频

An Integrated Approach for Microprotein Identification and Sequence Analysis

An Integrated Approach for Microprotein Identification and Sequence Analysis

Published on: July 12, 2022

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames

Published on: April 11, 2019

相关概念视频

Multi-species Conserved Sequences

Multi-species Conserved Sequences

Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...

Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

Signal Sequences and Sorting Receptors

Signal Sequences and Sorting Receptors

Signal sequences are short amino acid sequences that guide newly synthesized proteins to their proper location within the cell. Classical signal sequences are fifteen to sixty amino acids long and present at the N-terminus of a polypeptide chain. Each signal sequence has a conserved segment of basic residues towards their N terminus, a hydrophobic core, and a C-terminus rich in polar residues. The C-terminus also contains a signal cleavage site and features a -3 -1 sequence motif. The -3-1...