下一代测序用于阐明男性生殖障碍中的精液微生物组
Rhianna Davies1, Suks Minhas2, Channa N Jayasena1
1Department of Metabolism, Digestion and Reproduction, Imperial College London, London W12 0HS, UK.
Medicina (Kaunas, Lithuania)
|January 23, 2024
概括
精液含有微生物群,可以影响男性生育能力. 精液中的特定细菌失衡可能与精子质量降低和DNA损伤增加有关,但需要更多的研究.
科学领域:
- 生殖生物学 生殖生物学
- 微生物组研究 微生物组研究
- 泌尿器科 泌尿器科 泌尿器科 泌尿器科
背景情况:
- 男性不孕症是一个日益严重的公共卫生问题,平均精子数量迅速下降.
- 人类精液微生物组,以前未被认可,现在被理解在生殖健康中发挥作用.
- 精液中的细菌存在可以触发免疫反应,导致反应性氧物种的产生和潜在的精子损伤.
研究的目的:
- 调查精液微生物组的组成及其与男性不孕症的关联.
- 探索精子细菌形状,反应性氧物种和精子DNA损伤之间的关系.
- 解决目前用于种子微生物组表征的方法的局限性.
主要方法:
- 利用下一代测序 (NGS) 技术来描述种子微生物群,因为传统培养和PCR方法的局限性.
- 分析了对保存的细菌集群的研究,以及与种子参数的潜在关联.
- 已知的方法异质性,小样本大小,在现有研究中缺乏严格的净化程序.
主要成果:
- 在各种研究中确定了种子微生物组内常见细菌的保存集群.
- 观察到,特定细菌的枯竭或过度代表可能与异常的精液参数有关.
- 在目前的研究中发现了显著的变异性和相互矛盾的发现,阻碍了最终的结论.
结论:
- 精子微生物组是男性不孕症的一个复杂因素,特定的细菌组成可能会影响精子健康.
- 目前的证据不足以指导关于精液微生物组的临床实践.
- 需要进行更大规模的标准化研究,以阐明精子微生物组在男性生殖健康和不孕症中的确切作用.
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