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茄子斑矮人病毒分离的遗传变异和进化分析来自西班牙的分离物

Ana Alfaro-Fernández¹, Rafael Taengua², Isabel Font-San-Ambrosio¹

¹Instituto Agroforestal Mediterráneo (IAM), Universitat Politècnica de València (UPV), 46022 Valencia, Valencia, Spain.

Plants (Basel, Switzerland)

|January 23, 2024

概括

西班牙的茄子斑矮人病毒 (EMDV) 显示基因多样性较低,这表明单一的引入事件. 再组合似乎很少发生,负选择塑造了病毒进化.

科学领域:

植物病毒学植物病毒学
分子进化分子进化
人口遗传学人口遗传学

背景情况:

茄子斑矮人病毒 (EMDV) 是影响Solanaceae作物的重要病原体.
了解EMDV的遗传多样性和种群结构对于疾病管理至关重要.

研究的目的:

分析来自西班牙的EMDV分离物的遗传变异和种群结构.
为了将西班牙的EMDV隔离与来自全球其他地区的EMDV隔离进行比较.
研究重组和选择在EMDV进化中的作用.

主要方法:

病毒基因N (核体) 和L (复制酶) 的遗传学分析.
同义和非同义替代率的比较.
基因分化 (Fst试验) 和核酸多样性分析.
从GenBank获取序列以进行国际比较.

主要成果:

来自西班牙的EMDV分离物形成了一个基因同质的群体,与来自德国和英国的分离物聚集在一起.
遗传学分析揭示了EMDV的三个主要类和四个子类.
重组事件很少发生,负选择是主要的进化力量.
有证据表明,在有限的基因流动下,EMDV只在西班牙一次引入.

结论:

西班牙的EMDV种群的特点是遗传多样性较低,可能发生单一引入事件.
在EMDV中,重组是罕见的,净化选择是普遍存在的.
在希腊和意大利观察到高多样性和基因流动的对比模式.

关键词:

阿尔法核性哈博病毒 (Alphanucleorhabdovirus melongenae) 是一种有毒的病毒.基因流是指基因的流动.负链RNA病毒的病毒.核酸多样性的核酸多样性人类的基因组学.再组合的复合方式.选择的选择选择的选择.

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