对氨酸基因的综合性分析提供了对类动物血统特异性家族进化的见解
Zhi Zou1, Li Zhang1,2, Yongguo Zhao1,3
1National Key Laboratory for Tropical Crop Breeding, Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions, Institute of Tropical Biosciences and Biotechnology/Sanya Research Institute of Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China.
Plants (Basel, Switzerland)
|January 23, 2024
概括
在Brassicales中,素基因进化揭示了新的古老类 (N和M) 和由基因组重复驱动的谱系特异性扩张. 大多数素基因表现出种子/胚胎表达,在相似对中观察到结构分歧.
科学领域:
- 植物分子生物学 植物分子生物学
- 进化基因组学是进化的基因组学.
- 农作物科学 农作物科学
背景情况:
- 油脂素 (OLE) 是脂质滴形成和稳定油作物种子中的关键结构蛋白.
- 尤迪科特 (Eudicot) 中的氨酸基团的进化历史,特别是在布拉西卡尔 (Brassicales) 类中,仍然不完全理解.
研究的目的:
- 为了研究Brassicales序列中的素基因家族的演变.
- 为了识别和描述新型的氨酸和它们的进化起源.
- 了解氨酸基因的表达模式和结构差异,与它们的进化历史相关.
主要方法:
- 从 10 种 Brassicales 品种中鉴定和对 98 种素基因进行基因分析.
- 基于互惠最佳匹配的同类分析将基因分配到分类和正统组.
- 转录分析以确定Arabidopsis thaliana和Carica papaya的表达模式.
主要成果:
- 发现了两种新型的素基,N (古老,在*Amborella trichopoda*中发现) 和M (古老,广泛分布).
- 将98个素基因划分为6个分类 (U,SL,SH,M,N,T) 和9个正统组.
- 鉴定了Brassicales的谱系特异性扩张,归因于全基因组重复 (WGDs) 和古代玛事件.
- 观察到大多数素基因的种子/胚胎/内主要表达,与Clade T的花朵偏好形成鲜明对比.
- 记录了对等的氨酸对的结构和表达差异,包括内部增益.
结论:
- 这项研究阐明了Brassicales中oleosin基因家族的复杂进化轨迹,揭示了古老的起源和血统特定的适应.
- 这些发现突出了WGDs在Brassicales内的氨酸基因家族扩张中的作用.
- 描述的表达模式和结构变异为未来对模型和非模型植物 (如Carica papaya) 中的油素的功能研究提供了基础.
相关概念视频
Cis-regulatory Sequences
9.9K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
9.9K
Exon Recombination
3.6K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon...
Exon shuffling follows “splice frame rules.” Each exon...
3.6K
Pleiotropy
40.5K
Pleiotropy is the phenomenon in which a single gene impacts multiple, seemingly unrelated phenotypic traits. For example, defects in the SOX10 gene cause Waardenburg Syndrome Type 4, or WS4, which can cause defects in pigmentation, hearing impairments, and an absence of intestinal contractions necessary for elimination. This diversity of phenotypes results from the expression pattern of SOX10 in early embryonic and fetal development. SOX10 is found in neural crest cells that form melanocytes,...
40.5K
Epistasis Analysis
5.0K
Although Mendel chose seven unrelated traits in peas to study gene segregation, most traits involve multiple gene interactions that create a spectrum of phenotypes. When the interaction of various genes or alleles at different locations influences a phenotype, this is called epistasis. Epistasis often involves one gene masking or interfering with the expression of another (antagonistic epistasis). Epistasis often occurs when different genes are part of the same biochemical pathway. The...
5.0K


