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介质细胞干细胞衍生的外体改善多克索鲁比诱导的心脏毒性

Sawdah A Ali¹, Dinender K Singla¹

¹Division of Metabolic and Cardiovascular Sciences, Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, FL 32816, USA.

Pharmaceuticals (Basel, Switzerland)

|January 23, 2024

概括

介质干细胞衍生的外生体 (MSC-Exos) 减少多克索鲁比诱导的热,这是一个涉及心脏毒性的细胞死亡途径. 这表明MSC-Exos是缓解化疗引起的心脏损伤的有希望的疗法.

科学领域:

心血管研究研究心血管研究
细胞生物学细胞生物学
药理学药理学是指药理学的学科.

背景情况:

doxorubicin (DOX) 化疗可以引起心脏毒性,导致心力衰竭.
目前对DOX诱导心脏毒性 (DIC) 的治疗方法有限.
介质干细胞衍生的外体细胞 (MSC-Exos) 显示出治疗心脏病的潜力,但它们对DOX诱导的火死的影响尚不清楚.

研究的目的:

为了研究MSC-Exos在改善DOX诱导的pyroptosis中的有效性,在体外.
阐明MSC-Exos施加其保护作用的分子机制.

主要方法:

用DOX对待H9c2细胞以诱导热致死.
随后进行了MSC-Exos的治疗.
免疫细胞化学,西部涂抹和RT-PCR用于分析.

主要成果:

MSC-Exos显著减少了HMGB1/TLR4轴的激活.
炎症酶形成 (NLRP3) 和 pyroptotic 标记物 (caspase-1,IL-1β,IL-18,GSDMD) 的数量有所减少.
在DOX处理的H9c2细胞中,MSC-Exos减弱了炎症诱导的烧.

结论:

在一个体外模型中,MSC-Exos有效地减轻了DOX诱导的热.
MSC-Exos代表了DIC的有希望的治疗策略,提供MSC的好处,没有传统干细胞治疗的缺点.

关键词:

DOX DOX 是一个多克斯.在MSC-Exosos中使用.这是一种炎症炎症炎症炎症.热灭症是什么?

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