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通过Coriolopsis gallica有效脱色聚酸染料Sirius Grey的Laccase-Mediator系统:过程优化和毒性评估

Héla Zouari-Mechichi¹, Jihen Benali¹, Abdulrahman H Alessa²

¹Laboratory of Biochemistry and Enzymatic Engineering of Lipases, National Engineering School of Sfax (ENIS), University of Sfax, Sfax BP1173 3038, Tunisia.

Molecules (Basel, Switzerland)

|January 23, 2024

概括

这项研究优化了真菌laccase用于去色Sirius灰色,一个多色染料. 改进的工艺显著降低了织废水中的染料度及其相关植物毒性.

科学领域:

生物技术是生物技术.
环境科学环境科学
工业微生物学工业微生物学

背景情况:

织工业的废水含有反性的多色染料.
传统的处理方法与复杂的染料结构作斗争.
聚色染料的生物降解比单色或二色染料研究较少.

研究的目的:

调查四种亚结合染料Sirius灰色的生物降解.
优化脱色条件,使用乳介质系统.
评估处理的染料溶液的植物毒性降低.

主要方法:

孤立的真菌菌株 *Coriolopsis gallica* BS9 用于乳糖生产.
使用含有乳糖酶和1-基二三醇 (HBT) 的无细胞超浮剂作为介质.
采用了Box-Benken设计,以优化pH,酶,HBT和染料度.

主要成果:

在最佳条件下达到87.56%的脱色产量和2.95%/分钟的速度 (pH5,1U/mL乳糖,1mM HBT,50 mg/L染料).
染料溶液的植物毒性降低,发芽指数 (GI) 从29%增加到80%.
证明了Sirius灰色色变色的laccase媒介系统的有效性.

结论:

来自*Coriolopsis gallica* BS9的乳糖酶媒介系统对降解多色素染料有效.
优化条件显著提高了染料去除效率,减少了对环境的影响.
这种方法提供了一个有前途的替代方案,用于处理织废水,污染了固染料.

关键词:

1-基二三醇的使用西里乌斯灰色的灰色.亚染料是一种染料.脱色处理脱色处理拉卡斯拉卡斯拉卡斯拉卡斯拉卡斯拉卡斯

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