果病毒是通过种子传播的,但可能不是垂直传播的
Anna Wunsch1, Bailey Hoff1,2, Mario Miranda Sazo3
1Plant Pathology and Plant-Microbe Biology Section, School of Integrative Plant Science, Cornell University, Geneva, NY 14456, USA.
Viruses
|January 23, 2024
概括
在受感染树木的种子中发现了六种果病毒,检测率从20%到96%. 然而,这些病毒并没有从消毒的种子传播给幼苗,这表明没有胚胎传播.
科学领域:
- 植物病毒学 植物病毒学
- 园艺科学 园艺科学
- 分子诊断学 分子诊断
背景情况:
- 果树 (Malus domestica) 容易感染许多病毒.
- 缺乏有关果感染病毒的种子传播能力的信息.
研究的目的:
- 调查六种常见的果病毒的存在和种子传播能力.
- 为了确定像ACLSV,AGCaV,ARWV2,ASGV,ASPV和CCGaV这样的病毒是否在果中的种子中传播.
主要方法:
- 使用逆转录聚合酶链反应 (RT-PCR) 和定量RT-PCR (RT-qPCR) 在果种子中检测病毒.
- 对果肉和果皮中病毒存在的分析.
- 种子生长实验使用表面消毒的种子来评估传播.
主要成果:
- 在未经处理的果种子中检测到六种果病毒 (ACLSV,AGCaV,ARWV2,ASGV,ASPV,CCGaV) 的高发病率 (20-96%).
- 在商用果的果肉,皮和种子中发现了果茎插孔病毒 (ASPV).
- 在1000多个从灭菌种子中种植的苗木中,没有发现ACLSV,ASGV或ASPV的可检测传播.
结论:
- 这项研究为感染果树的几种病毒的种子传播性提供了第一个证据.
- 虽然病毒存在于果种子中,但在测试条件下,通过胚胎组织的传播似乎是可以忽略的或不存在的.
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