相关实验视频

Updated: Jul 11, 2026

The Portable Chemical Sterilizer PCS, D-FENS, and D-FEND ALL: Novel Chlorine Dioxide Decontamination Technologies for the Military

The Portable Chemical Sterilizer PCS, D-FENS, and D-FEND ALL: Novel Chlorine Dioxide Decontamination Technologies for the Military

Published on: June 29, 2014

评估Mpox病毒的稳定性,非活化和消毒有效性

Yuwei Li^1,2, Shiyun Lv^1,2, Yan Zeng^1,2

¹Wuhan Institute of Biological Products Co., Ltd., Wuhan 430200, China.

|January 23, 2024

概括

莫波克斯病毒 (MPXV) 在低温和冷解周期中稳定. 热和β-propiolactone有效地使MPXV无活化,而乙醇和Micro-Chem Plus等消毒剂则适用于表面消毒.

科学领域:

病毒学病毒学
传染性疾病传染性疾病
公共卫生公共卫生

背景情况:

自2022年5月以来的Mpox病毒 (MPXV) 爆发需要强有力的安全措施.
可靠的消毒和无活化协议对于处理传染性MPXV样本至关重要.

研究的目的:

评估MPXV在各种存储条件下的稳定性.
评估不同物理和化学剂对MPXV无活化和消毒的有效性.

主要方法:

MPXV 培养物受到不同的温度和结解周期的影响.
使用热 (56°C,70°C,95°C),β-propiolactone (BPL),Micro-Chem Plus (MCP) 和乙醇进行了无活化测试.
使用50%细胞培养感染剂量 (CCID50) 试验测量病毒标位.

主要成果:

MPXV在较低的温度下以及通过多个冷解周期 (稳定性高达12个周期) 显示出显著的稳定性.
通过热处理 (56°C40分钟,70°C10分钟,95°C5分钟) 和BPL (1:1000比) 实现了有效的无活化.
在接触1分钟后,MCP (2-5%) 和75%的乙醇使MPXV失活>4.25毫克.

结论:

由于MPXV具有相当大的稳定性,因此需要验证的失活方法.
热和BPL是MPXV完全失活的有效方法.
MCP和乙醇提供有效的消毒,增强MPXV处理的实验室安全协议.

关键词:

BPL BPL BPL BPL BPL BPL BPL BPL BPL BPL BPL BPL BPL BPL BPL BPL BPL BPL BPL BPL BPL BPL BPL BPL BPL BPL MPXVMPXV MPXVVMPXVMPXVMPXVMPXVMPXVMPXVMPXVMPXVMPMPXVMPXVMPXVMPXVMPXVMPXVMPXVMPXVMPXVMPXVMPXVMPXVMPXVMPXVMPXVMPXVMPXVMPXVMPXVMPXVMPXVMPXVMPXVMPXVMPXVMPXVMPXVMPXVMPXVMPXVMPXVMPXVMPXVMPXVMPXVMPXVMPXVMPXVMPXVMPXVMPXVMPXVMPXVMPXVMPXVMPXVMPXVMPX 消毒消毒消毒消毒热量热量热量热量热量没有激活的失活.稳定的稳定性稳定的稳定性

更多相关视频

Determining Viral Disinfection Efficacy of Hot Water Laundering

Determining Viral Disinfection Efficacy of Hot Water Laundering

Published on: June 21, 2022

Evaluation of the Efficacy of Organic Peroxyacids for Eradicating Dairy Biofilms Using an Approach Combining Static and Dynamic Methods

Evaluation of the Efficacy of Organic Peroxyacids for Eradicating Dairy Biofilms Using an Approach Combining Static and Dynamic Methods

Published on: December 9, 2022

相关概念视频

Physical Methods for Controlling Microbial Growth: Radiation and Filtration

Physical Methods for Controlling Microbial Growth: Radiation and Filtration

Radiation and filtration are essential tools for microbial control, targeting microorganisms through distinct mechanisms. Radiation eliminates microbes by damaging their DNA, either killing them or inhibiting their growth. Based on wavelength, radiation is classified into two types: nonionizing and ionizing radiation.Non-ionizing radiation, such as UV radiation (200–400 nm), is absorbed by DNA, causing defects that effectively disinfect surfaces, air, and water, including safety cabinets.

Chemical Agents for Microbial Control

Chemical Agents for Microbial Control

Chemicals play important roles in controlling microbial growth by targeting microbial structures and functions as sanitizers, antiseptics, disinfectants, and sterilants.Alcohols are commonly used sanitizers, effectively disrupting lipid membranes, which compromises cell integrity. They are also used as antiseptics and disinfectants due to their rapid action and versatility.Phenols and their derivatives phenolics , known for denaturing proteins and disrupting cell membranes, are particularly...

Antimicrobial Effectiveness

Antimicrobial Effectiveness

The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...