纤维细胞生长因子信号调节牙根的发展
Chengxue Jin1,2, Noritaka Adachi1, Yuki Yoshimoto1
1Department of Molecular Craniofacial Embryology and Oral Histology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), Tokyo, Japan.
Journal of anatomy
|January 23, 2024
概括
纤维细胞生长因子18 (FGF18) 促进了小鼠牙根的发育和牙周组织的形成. FGF18通过影响牙周组织化来调节根生长.
科学领域:
- 发育生物学 发展生物学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 纤维细胞生长因子 (FGF) 信号传递对组织形态发生,包括牙发育至关重要.
- 虽然FGF在牙冠发育中的作用已经确立,但其在牙根发育中的功能仍然在很大程度上未知.
- FGF18是硬组织形成的潜在调节者.
研究的目的:
- 研究FGF18在小鼠牙根发育中的作用.
- 阐明FGF18影响根延伸和牙周组织形成的机制.
主要方法:
- 用FGF18浸泡的肝素珠在出生后第6天 (P6) 植入小鼠第一牙芽中,并移植到囊下.
- 在现场杂交被用来分析Fgf18转录本地化.
- 在实验室中使用牙纸干细胞的研究评估了FGF18对细胞增殖和骨质生性标志物表达的影响.
- 对移植的牙的分析检查了特定基因 (Sp7,Bsp,Periostin) 的表达.
主要成果:
- 在移植的牙中,FGF18显著增强了根延长和牙周组织的形成.
- 在特定的发育阶段,检测到Fgf18转录在牙髓,Hertwig的上皮根盖和牙毛囊细胞中.
- 在体外,FGF18抑制了牙髓干细胞的增殖,并降低了关键的骨质生殖标志物 (Runx2,Alpl,Sp7).
- 应用FGF18可提高牙顶端介质中的Periostin表达,但不能诱导Sp7或Bsp的表达.
结论:
- FGF18在调节小鼠牙根发育方面发挥着重要作用.
- FGF18似乎通过调节牙周组织化来促进根部发育,而不是直接诱导骨质生成.
- 这些发现突出了FGF18作为牙根形成复杂过程中的关键因素.
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