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莫格罗斯V通过调节miR-21-5P/SPRY1轴来减轻炎症反应
Mengjie Han1, Haiping Liu1,2, Guoxiang Liu1
1Department of Pharmacy, Guilin Medical University, Guilin 541199, P.R. China. juanlovelife@163.com.
Food & function
|January 23, 2024
概括
天然甜味剂Mogroside V通过降低miR-21-5p的调节和提高SPRY1.1的调节来缓解肺炎. 这种天然化合物显示出针对miR-21-5p/SPRY1通路的抗炎治疗的前景.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 莫格罗斯V (MV),一种来自*Siraitia grosvenorii*的天然甜味剂,具有抗炎性质.
- 微RNAs (miRNAs) 与天然化合物的抗炎机制有关.
- 特定的miRNA参与和MV抗炎作用的分子途径在很大程度上仍未被阐明.
研究的目的:
- 确定关键的miRNAs调解Mogroside V.的抗炎作用.
- 阐明MV抗炎作用的潜在分子机制.
- 研究miR-21-5p/SPRY1轴在MV介导的炎症抑制中的作用.
主要方法:
- 使用卵胺 (OVA) 诱导的喘小鼠模型和用脂聚糖 (LPS) 处理的RAW 264.7细胞.
- 进行了miRNA测序 (miRNA-seq) 和mRNA测序 (mRNA-seq) 进行了全面分析.
- 关键的炎症媒介 (细胞因子,NO,ROS) 和蛋白质表达 (p-P65/P65,COX-2,iNOS) 被量化.
主要成果:
- MV显著降低了喘小鼠的肺炎和LPS治疗细胞的炎症反应.
- MV调低了miR-21-5p的表达和调高了SPRY1的表达,被确定为直接目标.
- miR-21-5p/SPRY1操纵实验证实了它们在调解MV的抗炎作用中的关键作用.
结论:
- 莫格罗斯V具有显著的抗炎作用,无论是在体外*还是体内*.
- 抗炎机制涉及miR-21-5p的下调和随后SPRY1.1的上调.
- miR-21-5p/SPRY1通路代表了Mogroside V在治疗炎症疾病中的潜在治疗标.
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