血液转录组分析揭示了COVID-19和妊娠前之间的基因交叉
Yijing Chu1, Min Li1, Mingze Sun1
1Department of Obstetrics, the Affiliated Hospital of Qingdao University, Qingdao, China.
Frontiers in immunology
|January 23, 2024
概括
这项研究确定了COVID-19和妊娠前之间的共同基因和途径,揭示了这两种疾病的潜在生物标志物和治疗点. 使用RT-qPCR验证了这些发现,证实了不同的基因表达特征.
科学领域:
- 基因组学和生物信息学
- 孕产妇和胎儿医学 孕产妇和胎儿医学
- 传染性疾病 传染性疾病
背景情况:
- 新冠病毒病2019 (COVID-19) 显著增加了全球死亡率.
- 孕前是一种与高孕产妇发病率和死亡率以及受限胎儿生长相关的妊娠并发症.
- 患有诸如孕前等血管疾病的孕妇可能更容易感染严重急性呼吸系统综合征冠状病毒2 (SARS-CoV-2) 感染.
研究的目的:
- 为了确定基因表达,调节器和COVID-19和产前之间的通路之间的共同点.
- 发现适用于COVID-19和产前症的新型治疗策略的潜在见解.
主要方法:
- 分析了COVID-19 (GSE152418) 和孕前 (GSE48424) 的转录组数据集.
- 使用Deseq2和limma包进行了差异基因表达分析.
- 确定了常见的差异表达基因 (DEG),随后进行了功能丰富,途径分析和转录因子/microRNA预测. 验证是在外围血液单核细胞 (PBMC) 上使用RT-qPCR进行的.
主要成果:
- 在COVID-19和产前的数据集之间发现了355个重叠的DEG.
- 基因本体学和KEGG通路分析揭示了这些条件之间的显著关联.
- 确定了10个枢纽基因 (例如MRPL11,ATP5I),RT-PCR证实了这两种疾病的不同表达特征.
结论:
- 该研究成功地确定了COVID-19和产前之间的共享DEG,功能途径和调节元素.
- 研究结果提供了对共享的分子机制和两种疾病的潜在治疗点的见解.
- 经过验证的枢纽基因表明,在COVID-19和孕前中,它们可能作为生物标志物或治疗点发挥作用.
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