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相关概念视频

Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

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Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
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Adherens Junctions01:24

Adherens Junctions

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Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types –  adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as  epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as  heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic
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Cell Adhesion Molecules - Types and Functions01:20

Cell Adhesion Molecules - Types and Functions

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Cell adhesion molecules (CAMs) are pivotal to multicellularity and the coordinated functioning of tissues and organ systems. They enable physical interactions between cells and provide mechanical strength to tissues. They also function as receptors for signal transmission across the plasma membrane. The CAMs are broadly classified into four families - integrins, cadherins, selectins, and immunoglobulin-like CAMs (IgCAMs).
CAM Families
The Integrin family of proteins is primarily  involved...
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Anchoring Junctions01:03

Anchoring Junctions

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Anchoring junctions are multiprotein complexes that help cells connect to other cells and the extracellular matrix. Anchoring junctions are present on the lateral and basal surfaces of cells, providing strong and flexible connections. Focal adhesions are often formed due to cell interactions with the ECM substrata, which initiate signal transduction via kinase cascades and other mechanisms. Together, they provide stability and tissue integrity. There are three types of anchoring junctions:...
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Immunoglobulin-like Cell Adhesion Molecules01:31

Immunoglobulin-like Cell Adhesion Molecules

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Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
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Tension Response at Adherens Junctions01:26

Tension Response at Adherens Junctions

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The adherens junctions that anchor cells together are multi-protein complexes that dynamically adapt to mechanical stimuli such as tensile forces and shear stress. Mechanosensory proteins in these junctions can sense such mechanical stimuli and undergo a shift in their conformation, resulting in an altered function — a process called mechanotransduction.
α-Catenin as a Mechanosensory Protein
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Assay of Adhesion Under Shear Stress for the Study of T Lymphocyte-Adhesion Molecule Interactions
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探索在精子生成过程中结合动态中的可溶性细胞间粘附分子-1的演变功能.

Xiang Xiao1,2, Yating Han1,3, Qin Li4

  • 1Center for Reproductive Health, School of Pharmaceutical Sciences, Hangzhou Medical College (Zhejiang Academy of Medical Sciences), Hangzhou, China.

Frontiers in endocrinology
|January 23, 2024
PubMed
概括

可溶性细胞间粘附分子-1 (sICAM-1) 削弱了血液丸屏障 (BTB) 并损害了精子的生产. sICAM-1可以通过影响SRC信号来调节BTB的透性,为男性不育提供潜在的治疗点.

关键词:
塞尔托利细胞是Sertoli细胞.血液-丸屏障 (BTB) 是一种疾病.细胞骨架 细胞骨架男人的生育能力 男人的生育能力可溶性细胞间粘附分子-1 (sICAM-1) 是一种溶性细胞间粘附分子.精子的产生是精子的产生.测试 测试 测试 测试

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Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes
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Assay of Adhesion Under Shear Stress for the Study of T Lymphocyte-Adhesion Molecule Interactions
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Using Cell-substrate Impedance and Live Cell Imaging to Measure Real-time Changes in Cellular Adhesion and De-adhesion Induced by Matrix Modification
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科学领域:

  • 生殖生物学 生殖生物学
  • 细胞生物学 细胞生物学
  • 免疫学 免疫学 免疫学

背景情况:

  • 细胞间粘附分子-1 (ICAM-1) 是免疫和上皮细胞上的一种糖蛋白.
  • 可溶性ICAM-1 (sICAM-1) 从细胞表面释放出来,与疾病的严重程度有关.
  • sICAM-1 调节血液丸屏障 (BTB) 和精子生成.

研究的目的:

  • 调查sICAM-1在调节BTB完整性和精子生成中的作用.
  • 探索sICAM-1影响BTB功能的分子机制.
  • 为了确定与BTB功能障碍相关的男性不孕症的潜在治疗点.

主要方法:

  • 在体外和体内对sICAM-1过度表达的研究.
  • 对BTB连接蛋白表达的分析 (N-cadherin, γ-catenin,连接素43).
  • 对SRC家族激酶 (SFK) 信号通路的评估.

主要成果:

  • sICAM-1的过度表达削弱了BTB,减少了连接蛋白,并导致生殖细胞丧失.
  • 膜结合ICAM-1表现出屏障强化作用,与sICAM-1形成鲜明对比.
  • sICAM-1 过度表达的 SRC 抑制,这表明 SRC 在 sICAM-1 的下游作用.

结论:

  • sICAM-1 作为一个分子开关,破坏了 BTB 完整性和 Sertoli-germ 细胞粘附.
  • SRC信号与sICAM-1对BTB动态的下游影响有关.
  • 准sICAM-1/SRC通路可能为治疗男性不孕症和障碍器功能障碍提供新的策略.