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分析了三例非结核菌真菌错误阳性PCR结果的病例
Wenwen Jin1, Jing Wang1, Xin Yang1
1The clinical Laboratory, Yantai Yuhuangding Hospital, 20 Yuhuangding East Road, Yantai 264000, Shandong, China.
Respiratory medicine case reports
|January 23, 2024
概括
实时PCR可以检测真菌菌,但由于序列重叠,可能会产生诺卡迪亚和其他细菌的错误阳性. 进一步的识别方法,如MALDI-TOF质谱仪,对于准确的诊断至关重要.
科学领域:
- 医学微生物学 医学微生物学
- 分子诊断学 分子诊断
- 传染性疾病 传染性疾病
背景情况:
- 实时光定量PCR (RT-PCR) 用于区分Mycobacterium结核病 (MTB) 和非结核病菌 (NTM).
- 在NTM和其他病原体 (如Nocardia otidiscaviarum) 之间重叠的序列可以导致RT-PCR中的异常放大曲线.
研究的目的:
- 调查使用RT-PCR检测NTM错误阳性结果的原因.
- 突出罕见病原体的替代鉴定方法的重要性.
主要方法:
- 三名患有呼吸道症状和肺部感染的患者的病例介绍.
- 用RT-PCR进行NTMDNA检测.
- DNA微阵列分析.
- 用于细菌识别的矩阵辅助激光脱/离子化飞行时间 (MALDI-TOF) 质谱仪.
主要成果:
- 在这三名患者中,RT-PCR表明NTM,但DNA微阵列显示没有非Mycobacterium结核病.
- MALDI-TOF MS发现了诺卡迪亚奥蒂迪斯卡维亚,Mycobacterium paratuberculosis,以及Mycolicibacterium fluoranthenivorans.这些细菌都被发现.
- 发现这些确定的细菌的序列与NTM原料和探针发生交叉反应,导致错误阳性.
结论:
- 在真菌菌体RT-PCR中异常放大需要进一步调查.
- 测序或MALDI-TOF MS对于避免错误识别和检测RT-PCR结果不确定的罕见病原体至关重要.
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