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Updated: Jul 5, 2025

Immunofluorescence Microscopy of γH2AX and 53BP1 for Analyzing the Formation and Repair of DNA Double-strand Breaks
10:47

Immunofluorescence Microscopy of γH2AX and 53BP1 for Analyzing the Formation and Repair of DNA Double-strand Breaks

Published on: November 3, 2017

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阿尔法-同核素调节黑色素瘤中核DNA双链断裂的修复

Moriah R Arnold1,2, Gabriel M Cohn3, Kezia Catharina Oxe4

  • 1Medical Scientist Training Program, Oregon Health and Science University, Portland, OR, USA.

bioRxiv : the preprint server for biology
|January 23, 2024
PubMed
概括

帕金森病蛋白α-synuclein (αSyn) 有助于黑色素瘤细胞的DNA修复,特别是在细胞核内. 它的缺失会使DNA损伤恶化,并阻碍恢复,影响癌症的进展.

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Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter06:59

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Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage10:44

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DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
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One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
The first step of...
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Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
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