出生时的甲基化概况与早期儿童肥胖有关
Delphine Lariviere1, Sarah J C Craig2,3, Ian M Paul3,4
1Department of Biochemistry and Molecular Biology, Penn State University, University Park, PA.
medRxiv : the preprint server for health sciences
|January 23, 2024
概括
新生儿表观遗传学,特别是带血和胎盘中的DNA甲基化,可以预测婴儿体重增加和肥胖风险. 这种甲基化风险评分识别了儿童肥胖风险较高的婴儿.
科学领域:
- 表观遗传学和发育生物学
- 儿科健康与营养学
- 基因组学和分子生物学
背景情况:
- 儿童肥胖是一个主要的全球健康问题,需要识别早期的风险因素.
- 表观遗传因素,如DNA甲基化,越来越多地被认为是导致肥胖风险的潜在因素.
- 了解生命早期的表观遗传特征对于开发有效的肥胖干预措施至关重要.
研究的目的:
- 调查出生时的DNA甲基化档案与六个月的婴儿体重结果之间的关联.
- 为了确定特定的基因,其甲基化模式预测婴儿体重增加,BMI和体重与长度比.
- 开发一种预测工具,用于识别儿童肥胖风险的婴儿.
主要方法:
- 使用Illumina Infinium MethylationEpic芯片对48名婴儿的带血液和胎盘样本进行全基因组DNA甲基化分析.
- 回归分析以确定可预测婴儿体重结果的甲基化概况.
- 将儿童/母亲健康和环境因素纳入分析.
主要成果:
- 在带血中确定了23个预测基因,在胎盘中确定了10个与婴儿体重结果相关的基因.
- 三个带血基因 (PLIN4,UBE2F,PPPP1R16B) 的甲基化概况与所有三种体重结果相关,并在独立的队列中得到验证.
- 开发了一种甲基化风险评分 (MRS) 来识别儿童肥胖风险较高的婴儿.
结论:
- 婴儿出生时的DNA甲基化模式与生命早期的体重轨迹和肥胖风险有很大关系.
- 特定的基因和一种新的MRS显示出早期识别和儿童肥胖的干预策略的前景.
- 需要进一步的功能性研究来阐明已识别的基因在肥胖发展中的作用.
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